耐多药结核分支杆菌基因突变在耐药性检测中的应用医学.docVIP

耐多药结核分支杆菌基因突变在耐药性检测中的应用医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：耐多药结核分支杆菌基因突变在耐药性检测中的应用医学 1 1 材料与方法 3 2 结果 4 3 讨论 4 文2：耐多药结核分枝杆菌基因突变在耐药性检测中的应用 5 1 材料和方法 6 2 结果 7 3 讨论 8 参考文摘引言： 8 原创性声明（模板） 10 文章致谢（模板） 10 正文 耐多药结核分支杆菌基因突变在耐药性检测中的应用医学 文1：耐多药结核分支杆菌基因突变在耐药性检测中的应用医学 Studies of Mycobacterium tuberculosis multi-drug resistant gene 【Abstract】 Objective To evaluat the importance of rPOB、KatG and rpsL gene mutation detection in multi-drug resistant clinical isolates of Mycobacterium tuberculosis. Methods rPOB、KatG and rpsL gene mutation of 54multi-drug resistant clinical isolates，which is INH RFP and Sm resistance and 54 drug susceptible strai were analyzed using polymerase chain reaction-single strand conformation (PCR-SSCP).Results SSCP pattern of H37RV strain as control，SSCP pattern of rPOB，rpsL PCR amplification from drug susceptible strai were all the same as control. The specificity was 100%. However，the different SSCP pattern of KatG gene were found in 2 susceptible strai. The specificity was %. Gene deletion of rPOB、KatG and rpsL were not found in all clinical isolates tested. SSCP patter of rPOB gene were different from control in 49 drug resistant clinical isolates. SSCP patter of KatG gene were different from control in 32drug resistant clinical isolates，SSCP pattern of rpsL gene were different from control in 40 drug resistant clinical isolates. The seitivity were % (rPOB)，% (KatG) and rpsl (%)，respectively. Conclusion The major mechanism of RFP，INH，SM resistant drug was rPOB、KatG and rpsL gene mutation，respectively PCR-SSCP may be one of the way which applied to drug resistant detection of Mycobacterium tuberculosis. 【Key words】 Mycobacterium tuberculosis；rPOB gene；KatG gene；rpsL gene；SSCP；drug tolerance 我国是结核病疫情最严重的国家之一，其主要原因是耐药（DR-TB）和耐多药（MDR-TB）结核病的广泛分布和迅速传播。由于单药化疗和不规则化疗，其耐药情况越来越严重。近年来随着分子生物学的研究进展人们对抗结核分枝杆菌的药理作用位点和结核分枝杆菌耐药机制，尤其是耐异烟肼、利福平和链霉素。目前，结核分支杆菌耐药性测定仍多采用绝对浓度法、比例法等经典方法。但由于结核分支杆菌生长缓慢，而耐药结核分支杆菌生长更为缓慢，其耐药性测定需6～8周甚至更长，不能及时指导