乳腺癌端粒酶活性与PCNA表达关系.docVIP

乳腺癌端粒酶活性与PCNA表达关系 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：乳腺癌端粒酶活性与PCNA表达关系 1 1 对象与方法 3 2 结果 4 3 讨论 5 文2：乳腺癌端粒酶活性与PCNA表达关系 6 1对象与方法 7 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 11 正文 乳腺癌端粒酶活性与PCNA表达关系 文1：乳腺癌端粒酶活性与PCNA表达关系 Relatiohip between telomerase activation and PCNA expression in breast cancer Abstract： Objective To approach the interrelation between telomerase activation and PCNA expression in breast cancer and evaluate the significance of combined detection in formulating therapeutic regimen after breast cancer The level of telomerase activation and PCNA expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry (1)The positive rate of telomerase in 80 breast cancer tissues and 36 side-cancer tissues were % and % respectively and the difference revealed statistical significance(P001),the telomerase activation was not examinated in 10 cases of lacteal gland benign lesion;It is no significance in statistic that the level of positive rate of telomerase and the extent of axillary nodes metastasis(P005).(2)Among 80 cases ,the positive rate of PCNA in breast cancer tissues was % and the positive rate of PCNA in group with lymphatic metastasis was significantly higher than that in group without lymphatic metastasis(P001) and there was positive relatiohip between lymphatic metastasis and positive expression of PCNA(P001).(3)The telomerase activation was in accordance with PCNA expression(P001) and there was positive relatiohip between telomerase activation and PCNA(P001),the concordance rate was %.Conclusion The telomerase activation is in accordance with PCNA expression,associating telomerase activation with PCNA detection can provide the ancillary evidence to predict the prognosis and to formulate the treatment plan after breast cancer operation. Key words： breast cancer;telomerase;proliferating cell nuclear antigen(PCNA);poly