葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响.docVIP

葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响 1 1 材料与仪器 3 2 方法 3 3 结果 4 4 讨论 6 文2：菩人丹胶囊对2型糖尿病大鼠胰岛功能的影响 7 1材料与仪器 7 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响 文1：葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响 Abstract：ObjectiveTo investigate the effect of grape seed procyanidin (GSPC) on renal functional protein of the type 2 diabetes mellitus (T2DM) model of T2DM was developed in male wistar rats. The diabetes rats were received GSPC (100 mg·kg-1·d-1) for 12 weeks. The following paramete were measured: the fasting blood sugar (FBG), blood fat (TG), total cholesterol (TC) level, KW/BW, iulin seitivity index (ISI), superoxide dismutase (SOD), nitric oxide (NO), nitric oxide synthase (NOS) and sodium pump activity of kidney proximal tubules (PT).Results①Compared with normal control group, FBG, TG, TC level and KW/BW in the model control group were significantly increased and SOD, NO, NOS, ISI and sodium pump activity were significantly decreased; ②Compared with model control group, TG, TC level and KW/BW in the GSPC treat group were significantly decreased while SOD, NO, NOS, ISI and sodium pump activity were significantly increased. ConclusionGSPC can protect renal function in T2DM rats by decrcasing oxidative stress． Key words：Procyanidin; Type 2 diabetes mellitus; Oxidative stress; Functional protein; Rats 葡萄籽原花青素(grape seed procyanidin, GSPC)是从葡萄籽中提取出来的一类属于双黄酮衍生物的天然多酚化合物。动物实验表明，GSPC具有抗氧化、抗炎、抗动脉硬化及保护心血管等作用［1］。目前研究认为糖尿病肾病(Diabetic Nephropathy, DN)的发生除了与高血糖有关外，也与糖尿病患者抗氧化应急能力下降［2］，炎症介质增加［3］，动脉粥样硬化有密切的联系。本实验室前期研究证实了糖尿病大鼠在尿液微球蛋白出现前，肾脏功能蛋白，特别是实现肾脏功能最关键的近端小管(proximal tubule, PT)管周膜上钠泵活性已经改变［4］，所以本实验用GSPC灌胃治疗T2DM大鼠，通过检测肾脏PT钠泵活性及血清中超氧化物歧化酶(superoxide dismutase, SOD)、一氧化氮(nitric oxide, NO)、一氧化氮合酶(nitric oxide synthase, NOS)等的变化，从而研究GSPC对T2DM大鼠肾脏功能蛋白的保护作用与机理。 1 材料与仪器 动物及饲料 3月龄雄性Wistar大鼠，一级，体质量(195±16) g。动物合格证号：SCXK(川)2004-15。常规饲料：57%碳水化合物，22%蛋白质，6%脂肪，8%纤维素，7%其他成分。高能饲料(60%常规饲料，20%蔗糖，15%猪油，5%胆固醇)。动物及饲料均由四川