复方鳖甲软肝方防治特发性肺间质纤维化的MMPs表达药学.docVIP

复方鳖甲软肝方防治特发性肺间质纤维化的MMPs表达药学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：复方鳖甲软肝方防治特发性肺间质纤维化的MMPs表达药学 1 1 器材 2 2 方法 4 3 结果 7 4 讨论 8 文2：特发性肺间质纤维化的中医研究 10 1中医对于特发性肺间质纤维化疾病的认知 10 2中医对于特发性肺间质纤维化疾病的治疗 11 3讨论 12 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 复方鳖甲软肝方防治特发性肺间质纤维化的MMPs表达药学 文1：复方鳖甲软肝方防治特发性肺间质纤维化的MMPs表达药学 Abstract:ObjectiveTo study the effect of compound prescription witch turtle shell (CPTS) on the expression of MMPS in rats with IPF (Idiopathic pulmonary intetitial fibrosis) caused by Bleomycin male SD rats were randomly divided into six groups:sham operation group, model group, positive medicine groups, high-dosage group, medium-dosage group and low-medium group. After they were given the medicine, ten rats in each group each time were killed respectively on the 7th day, 14th day and 28th day. MMP-2 was detected by in situ hybridization and RT-PCR, MMP-9 by immunohistochemical method. ResultsCPTS could inhibit expression of MMP-9 in early phase and MMP-1 in middle and late phases. Result of hybridization in situ of MMP-2 was similar to that of RT-PCR. The effects of medium dose groups and low dose were better. ConclusionCPTS can inhibit the expression of MMP-9 in early phase and MMP-1 in middle and late phases. CPTS has a protective effect on IPF by inhibiting the expression of MMP-9. Key words:Compound Prescription witch turtle shell; IPF; MMPs 细胞外基质（extra cellular matrix , ECM）蛋白与其它类型的蛋白一样，有合成过程也有降解过程。ECM蛋白翻译以后的修饰加工过程，以及蛋白质的降解过程，都是由酶来催化的，这些蛋白酶类属于锌内肽酶，因而称之为基质金属蛋白酶（matrix metal proteinases,MMPs ）。MMPs 来源于多种类型的细胞，其在ECM（extra cellular matrix ）破坏和降解过程中具有十分重要的作用。本文主要对基质金属蛋白酶在特发性肺间质纤维化表达进行实验研究。 1 器材 药物 防治药物复方鳖甲软肝方（内蒙古福瑞中蒙药科技股份有限公司生产的复方中药干粉，600 g/瓶，批号：）。高剂量 g·kg-1·d-1、中剂量 g·kg-1·d-1、低剂量药物为 g·kg-1·d-1。相当于临床日用量（ g·kg-1）的14倍，7倍，倍。 阳性对照药醋酸强的松 mg(100 g-1·d-1）广东华南制药厂生产，批号011201。 造模药物注射用盐酸博莱霉素（bleomycin，BLM-A5），日本化药株式会社制造，批号X12100。 动物SD大鼠180只，雄性，体重200～220 g，购于北京维通利华实验动物技术有限公司，动物级别：SPF/VAF，许可证编号：SCXK2002-0003。饲养于同级动物房，自由饮水。购入后适应性饲养1周，进行实验分组。随机分为6组: