Ghrelin对MPTP小鼠帕金森病模型黑质THBcl2和BaxmRNA表达的影响医学（行业资料）.docVIP

Ghrelin对MPTP小鼠帕金森病模型黑质THBcl2和BaxmRNA表达的影响医学（行业资料） 文档信息 主题： 关于“行业资料”中“医学资料”的参考范文。 属性： F-01L7FG，doc格式，正文29858字。质优实惠，欢迎下载！ 适用： 作为医学资料、医学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：Ghrelin对MPTP小鼠帕金森病模型黑质THBcl2和BaxmRNA表达的影响医学 2 1 材料与方法 3 2 结 果 5 3 讨 论 6 文2：JNK通路对亚急性帕金森病MPTP模型小鼠黑质COX2表达的影响医学 8 1材料和方法 9 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 Ghrelin对MPTP小鼠帕金森病模型黑质THBcl2和BaxmRNA表达的影响医学（行业资料） 文1：Ghrelin对MPTP小鼠帕金森病模型黑质THBcl2和BaxmRNA表达的影响医学 ［ABSTRACT］ObjectiveTo investigate the effect of Ghrlein on tyrosine hydroxylase (TH), Bcl2 and Bax mRNA expression of the subatantia nigra in 1methy4phenyl1,2,3,6tetrahydropyridine (MPTP)induced mouse model of Parkion disease. MethodsEighteen healthy male C57BL/6 mice were divided into three groups at random：Control, MPTP and Ghrelin+MPTP. The mice in MPTP group were injected with MPTP (30 mg/kg, .), once daily for five days, with Ghrelin (.), 2 h before the injection of MPTP, once daily for eight days. As for the Control group, MPTP and ghrelin were replaced by normal saline. The total mRNA of the substantia nigra (SN) was collected to detect the mRNA expression of TH, Bcl2 and Bax by semiquantitive revee tracription polymerase chain reaction. ResultsCompared with the control group, MPTP induced a reduction of TH mRNA expression (P). Pretreated with ghrelin showed an increase of TH mRNA expression compared with MPTP group (P). MPTP also decreased the ratio of Bcl2/Bax to ± (P). And ghrelin could revee the reduction induced by MPTP (P). ConclusionGhrelin could inhibit the reduction of TH mRNA expression induced by MPTP. The antiapoptotic pathway might be the mechanism of this neuroprotective effect. ［KEY WORDS］Ghrelin; Parkion disease; 1methy4phenyl1,2,3,6tetrahydropyridine; tyrosine 3monooxygenase; apoptosis 帕金森病（PD）是一种常见的中枢神经系统退行性疾病，其病理改变主要是中脑黑质（SN）致密带（SNpc）多巴胺（DA）能神经元死亡。PD的病因未完全明了，可能有多种因素参与，如遗传、环境因素、氧化应激、兴奋性毒素、自身免疫、SN铁沉积、细胞凋亡等[1]。近年来研究发现，细胞凋亡在PD 发病中起着