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台灣質譜學會93年度教育訓練課程 －質譜分析技術 93/4/9 (五)串聯質譜儀(Tandem-MS) (二) – TOF instrument廖寶琦成功大學環境醫學研究所What is Tandem Mass Spectrometry?Dictionary definition of “tandem”:An arrangement of two or more people or things placed one behind the other.For examples, tandem bicycleTandem Mass Spectrometry:An arrangement of two or more mass spectrometers (tandem-in-space) or mass spectrometry experiments (tandem-in-time) placed one behind the other.Acronym: MS/MS; MS3; MSn A+C+D+B+m/zA2+A4+A1+IA3+A+m/zAn Example of Tandem Mass Spectrometry Experiment: Product Ion Scananalytes A, B, C, Dionization sourceIA+ , B+ , C+ , D+mass analyzer IA+precursor ion fragmentationA1+ , A2+ , A3+ , A4+product ionsmass analyzer IIActivation (Dissociation, Fragmentation) Methods in MS/MSMetastable decayCollision-induced dissociation (CID), or collisionally activated dissociation (CAD). High energy vs. low energy CID.Surface induced dissociation, photo-dissociation, electron excitation.Rationales for (Why) PerformingTandem Mass SpectrometryTo obtain chemical structure-related information. For example, differentiation of isobar compounds or isomers.To increase selectivity and S/N ratio in quantitative measurement. For example, metabolite quatitation in complex biological matrices.MS/MS by fragmentation enhances selectivityisobarMW: 278 Da for bothdistinct fragmentsFragment Ions Derived from N7-HEGby CID-MS/MS experiment ProductIonPrecursorIonHigh Sensitivity and Specificity Provided by LC-MS/MS for N7-HEG DetectionWithout matrixMatrix effect considered4 fmol16 fmolLC-MS/MS128 fmol1.02 pmolLC-MSHigh Sensitivity and Specificity Provided by LC-MS/MS for N7-HEG DetectionWithout matrixMatrix effect considered128 fmol1.02 pmolLC-MS/MS128 fmol1.02 pmolLC-MSCommon Applications ofTandem Mass SpectrometryExamination of ion-molecule reactionsMolecular structural confirmationPharmacokinetic investigationsNeonatal screening for newborn diseasesAbused drug screening when LC-MS is usedBiological monitoring of toxicant exposure P