黄芪对哮喘大鼠肺组织p38蛋白激酶表达的影响（临床医学范文）.docVIP

黄芪对哮喘大鼠肺组织p38蛋白激酶表达的影响（临床医学范文） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：黄芪对哮喘大鼠肺组织p38蛋白激酶表达的影响 2 1 材料和方法 3 1.1 大鼠哮喘模型的建立和分组 3 1.3 BALF细胞涂片、细胞计数和分类 4 1.4 肺组织磷酸化p38 MAPK表达的检测 4 1.5 肺组织病理学观察 4 1.6 统计学处理 5 文2：黄芪对哮喘大鼠肺组织p38蛋白激酶表达的影响 5 1 材料和方法 6 1.1 大鼠哮喘模型的建立和分组 6 1.3 BALF细胞涂片、细胞计数和分类 7 1.4 肺组织磷酸化p38 MAPK表达的检测 7 1.5 肺组织病理学观察 8 1.6 统计学处理 8 2 结 果 8 2.1 各组大鼠BALF中EOS计数和IL5含量的变化 8 2.2 肺组织磷酸化p38 MAPK表达的变化 8 2.4 肺组织病 理学 改变 9 3 讨 论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 黄芪对哮喘大鼠肺组织p38蛋白激酶表达的影响（临床医学范文） 文1：黄芪对哮喘大鼠肺组织p38蛋白激酶表达的影响 ABSTRACT Objective To explore the effect of astragalus injection on the expression of p38 mitogenactivated protein kinase (p38 MAPK) in asthmatic rats. Methods Ovalbumin (OVA) was injected intraperitoneally and inhaled to produce the asthmatic model. Twentyfour rats were randomly divided into three groups: control group, asthma group and astragalus group. The concentration of IL5 in BALF and the expression of phosphop38 MAPK in lung tissue were measured by ELISA and Western blot, respectively. Results The expression of phosphop38 MAPK was upregulated in the lung tissue of asthmatic rats. Compared with asthma group, astragalus injection significantly decreased the expression of phosphop38 MAPK , the concentration of IL5 and the number of eosinophil in BALF (P0.01), and alleviated the histopathological damage of lung tissue. There were positive correlatio between the expression of phosphop38 MAPK and the concentration of IL5 and the number of eosinophil in BALF. Conclusion p38 MAPK may play a role in pathological process of asthma. Astragalus could effectively treat asthma by inhibiting the expression of p38 MAPK. KEY WORDS： Asthma；p38 mitogenactivated protein kinase; Astragalus 新近研究表明，p38丝裂原活化蛋白激酶（p38 mitogenactivated protein kinase，p38 MAPK）是细胞内重要的信号传递者，参与了对多种炎症细胞因子和多种类型的细胞应激信号的传导，在哮喘的发生和发展过程中有着重要作用[1，2]。黄芪作为传统中药，可通过降低患者气道高反应性[3]，调节患者免疫功能[4],从而对支气管哮喘有一定的治疗作用。本研究通过观察黄芪对哮喘大鼠肺组织p38