两种染色方法在细胞核DNA含量检测中的应用及比较（临床医学范文）.docVIP

两种染色方法在细胞核DNA含量检测中的应用及比较（临床医学范文） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：两种染色方法在细胞核DNA含量检测中的应用及比较 1 1 材料与方法 3 1.2 快速Feulgen染色 3 1.3 改良法Feulgen染色 3 1.4 图像分析仪的校正与肝细胞核参数的测量 4 文2：两种染色方法在细胞核DNA含量检测中的应用及比较 4 1 材料与方法 6 1.2 快速Feulgen染色 6 1.3 改良法Feulgen染色 6 1.4 图像分析仪的校正与肝细胞核参数的测量 6 2 结果 7 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 11 文章致谢（模板） 11 正文 两种染色方法在细胞核DNA含量检测中的应用及比较（临床医学范文） 文1：两种染色方法在细胞核DNA含量检测中的应用及比较 Application and comparison of two kinds of staining methods for Abstract: Objective To find out the suitable hydrolysis time for two staining methods of Feulgen and to provide guidline on research and clinic. Methods Smea of liver cell suspeion were obtained from five healthy rats. All the hepatocytes are, respectively, hydrolyzed in different time at room temperature and 60℃, and then stained with modified Feulgen stain and quick one. DNA content and ploid of intact hepatocyte nuclei was analyzed by TIGER cell image analysis system. Results (1) the nuclear DNA contents are almost identical from same DNA content ploidy of different rats under the same temperature and time of hydrolysis, and the CV of IOD from three types of DNA content ploidy was less than 10 percent. (2) The IOD ratio among diploid, tetraploid and octaploid was close to 2 and 4. (3) The DNA contents of hepatocyte nuclei from same DNA content ploidy of different rats were distinct under the same temperature and different hydrolysis time: hydrolysis at 60℃, IOD(5～7 min)IOD(9～15 min)IOD(1 min，20 min); hydrolysis at room temperature, IOD(50 min)IOD(20～30 min，70～90 min)IOD(5～10 min，100 min). Conclusion nuclei DNA cant be stained better for extra long or short hydrolysis time, the better hydrolysis time of quick Feulgen stain and modified one are, respectively, 5～7 min and 20～90 min. Key words: DNA analysis; staining and labeling methods; feulgen staining; smear 细胞核的DNA含量测量与倍体分析对判断肿瘤的良恶性及预后有重要参考价值［1～4］。自1924年Feulgen和Rossenbeck［5］在组织原位上用Feulgen反应显示细胞核的DNA以来，Feulgen反应已广泛应用于生物医学、药学