热化疗联合对人肺腺癌耐药细胞株A549DDP作用及其机制的实验研究（临床医学范文）.docVIP

热化疗联合对人肺腺癌耐药细胞株A549DDP作用及其机制的实验研究（临床医学范文） 文档信息 ： 文档作为关于“医学心理学”中“肿瘤学”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文9542字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：热化疗联合对人肺腺癌耐药细胞株A549DDP作用及其机制的实验研究 2 1 材料与方法 3 1.1 材料 3 1.2 方法 3 1.3 统计学处理 5 文2：热化疗联合对人肺腺癌耐药细胞株A549DDP作用及其机制的实验研究 5 1 材料与方法 6 1.1 材料 6 1.2 方法 7 1.3 统计学处理 8 2 结果 8 2.2 热疗和/或化疗对A549/DDP细胞周期的影响 9 3 讨论 9 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 热化疗联合对人肺腺癌耐药细胞株A549DDP作用及其机制的实验研究（临床医学范文） 文1：热化疗联合对人肺腺癌耐药细胞株A549DDP作用及其机制的实验研究 【Abjuctive】 Objective To observe the effects and mechanisms of hyperthermia therapy combined with chemotherapy on multidrug-resistant human lung adenocarcinoma cell line A549/DDP, it will provide theory evidence in thermochemotherapy for clinic. Methods A549 or A549/DDP treated with different temperature were detected of seitivity to cisplatin with CCK-8 assay. After hyperthermia, A549/DDP cell cycle was analyzed with flow cytometry and ERCC1mRNA expression was detected using RT-PCR. Results Parent human lung adenocarcinoma cell A549 was more seitive to cisplatin than drug-resistant cell line A549/DDP. Treated with different temperature, drug seitivity of A549/DDP to cisplatin all increased. Hyperthermia had significant effect on cell cycle with increase of G2/M phase population and reduction of G0/G1 phase population, and thermochemotherapy was more notable（P0.01）. After hyperthermia, the expression of ERCC1 mRNA did not change. Conclusion Hyperthermia can significantly enhance the seitivity to cisplatin in human multidrug-resistant lung cancer cell line A549/DDP. Thermochemotherapy has synergistic effects of inhibiting cell proliferation. The major synergistic mechanisms of thermochemotherapy are that they can induce cell cycle arrest、inhibit cell proliferation ,but have no correlation with gene ERCC1. 【Key words】 Hyperthermia Chemotherapy Lung cancer Drug resistance 肺癌是人类最常见的恶性肿瘤之一，即使最佳的联合治疗，非小细胞肺癌（nomall-cell lung cancer, CLC