大鼠酒精性肝病模型的建立（临床医学范文）.docVIP

大鼠酒精性肝病模型的建立（临床医学范文） 文档信息 ： 文档作为关于“医学心理学”中“内科学”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文8720字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 2 文1：大鼠酒精性肝病模型的建立 2 1 材料与方法 3 1.1 材料 3 1.2 动物模型的建立 4 1.3 观察指标及测定方法 4 1.4 统计学分析 4 2 结果 5 2.1 一般情况 5 文2：大鼠酒精性肝病模型的建立 5 1 材料与方法 7 1.1 材料 7 1.2 动物模型的建立 7 1.3 观察指标及测定方法 8 1.4 统计学分析 8 2 结果 8 2.1 一般情况 8 2.2 肝组织病 理学 改变 8 2.3 肝功能变化 9 2.4 肝脏超微结构改变 9 3 讨论 9 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 大鼠酒精性肝病模型的建立（临床医学范文） 文1：大鼠酒精性肝病模型的建立 【Abstract】 Objective To induce a relatively simple and feasible rat model of alcoholic liver disease by the freely drinking and perfusing stomach with alcohol, which is close clinical situation and timesaving . Methods Rat model of alcoholic liver disease was induced by the freely drinking and perfusing stomach with alcohol .The rats were intragastric administratered in fit with a special amount of alcohol receiving alcohol drinking water as the sole source of liquid. The alcohol concentration was increased from 40%( alcohol /water: v/v)to 45% and to a final concentration of 50% for 4,4,2 weeks by a tube everyday .Then the modal group drank alcohol freely from the 11th week .In the control group, rats were intragastric administratered with equal in early 10 weeks,and drunk water freely from the 11th week. After 12 weeks, almandine aminotraferase (ALT), aspartateaminotraferase(AST), albumin(ALB),albumiglobulin(A/G) of rats drinking and perfusing stomach with alcohol were detected and were compared with those of the control rats.Used t -test for comparative method of two sample mea. And the liver tissues were observed both in light microscope（LM） and electron microscope（EM）. Results After 12 weeks, AST of rats drinking and perfusing stomach with alcohol were increased, ALB and A/G were decreased, compared with those of the normal rats, and alcohol hepatitis were aggravated and fibrosis were observed in pathology and ultrastucture