健骨颗粒通过G1S期调控蛋白对成骨细胞增殖的影响（基础医学范文）.docVIP

健骨颗粒通过G1S期调控蛋白对成骨细胞增殖的影响（基础医学范文） 文档信息 ： 文档作为关于“建筑或环境”中“保温建筑”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文13297字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 2 文1：健骨颗粒通过G1S期调控蛋白对成骨细胞增殖的影响 2 1.1 实验药物 3 1.2 实验动物 4 1.3 实验试剂 4 1.4 仪器与设备 4 1.5 方法 5 1.6 统计学方法 6 2 结果 6 2.1 不同浓度含药血清干预后成骨细胞增殖情况 6 2.3 不同含药血清干预后成骨细胞细胞周期的分布 7 3 讨论 8 文2：强磁重力环境对成骨细胞增殖形态及其主要元素含量的影响 12 1材料和方法 13 1.2方法 13 2.2HMGE对细胞形态的影响 15 2.3HMGE对细胞中主要元素含量影响 16 参考文摘引言： 18 原创性声明（模板） 19 文章致谢（模板） 20 正文 健骨颗粒通过G1S期调控蛋白对成骨细胞增殖的影响（基础医学范文） 文1：健骨颗粒通过G1S期调控蛋白对成骨细胞增殖的影响 [Abstract] Objective To observe the effects of Jiangu Granules on the G1 phase cell-cycle regulated protei of osteoblasts， so as to reveal the mechanism of Jiangu Granules in promoting osteoblasts proliferation. Methods The Jiangu Granules-serum group， model-serum group and estradiol-serum group were prepared. Osteoblasts of SD rats were cultivated by enzymatic digestion and intervened with Jiangu Granules-serum， model-serum and estradiol-serum （as control） respectively. The cell cycles were analyzed by flow cytometry， while the fluorescence quantitative PCR was applied to measure cyclin E， CDK2， p21 and E2F-1 mRNA. Results The cell proliferation rates of osteoblasts intervened with estradiol serum and Jiangu Granules-serum for 48 hou were faster than that with the same concentration model-serum （P 0.01）. Compared with the model-serum group， the proportion of osteoblasts in the G0/G1 phase were significantly reduced after intervention with 15% of estradiol serum and Jiangu Granules-serum for 48 hou （P 0.01）， while the proportion of cells in S phase， G2/M phase and proliferation index was much higher （P 0.01）. The expression of cycling E， CDK 2 and tracription factor E2F-1 mRNA of osteoblasts in the Jiangu Granules-serum group and the estradiol-serum group was higher than that of model-serum group （P 0.01）， while the expression of p21 mRNA was lower than the model-serum group （P 0.01）. C