三叉苦黄酮及生物碱类成分的薄层色谱分析最终稿.docxVIP

三叉苦黄酮及生物碱类成分的薄层色谱分析

【摘要】目的：本实验建立三叉苦药材黄酮及生物碱类成分的高效薄层色谱指纹图谱（HPTLC）的分析方法，为三叉苦质量标准制定提供参考。方法：分别提取三叉苦药材的黄酮及生物碱类成分，进行理化显色，采用紫外分光光度法初步测定总黄酮含量。以汉黄芩素、吴茱萸春碱为标准品，采用高效薄层色谱法，控制相对湿度为67%，温度25℃，生物碱在氯仿-甲醇-乙酸乙酯（10:1:0.2）的展开剂下展开和5%茚三酮溶液中显色，黄酮类化合物在甲苯-氯仿-乙酸乙酯-乙酸（7:1:1.8:0.2）的展开条件下进行展开、5%三氯化铝乙醇溶液中显色，于365nm紫外光下拍照观察，对不同批次三叉苦的高效薄层色谱图用Scanner色谱软件进行分析。结果：以汉黄芩素为标准品，测定总黄酮的含量范围在4.20mg/g~14.95mg/g之间，其薄层色谱图由3个特征斑点构成，其中汉黄芩素的Rf值约为0.46。以吴茱萸春碱为标准品，建立了由7个特征斑点构成的薄层色谱图，其中吴茱萸春碱的Rf值约为0.67。通过HPTLC指纹图谱考察，不同批次的三叉苦药材质量参差不齐。结论：此方法简单易行，可有效鉴别三叉苦，并评价其质量。

【关键词】三叉苦；高效薄层色谱指纹图谱；黄酮；生物碱

ThinLayerChromatographicAnalysisofFlavonoidsandAlkaloidsinMelicopePteleifolia

[Abstract]OBJECTIVEToestablishahighperformancethin-layerchromatography(HPTLC)fingerprintmethodforthedeterminationofflavonoidsandalkaloidsinMelicopepteleifolia,andprovidereferenceforthequalitystandardofMelicopepteleifolia.METHODSExtractingtheflavonoidsandalkaloidsfromTrigeminalbitterness,andhavingphysicalandchemicalidentificationandcolordevelopment,anddoingapreliminaryexaminationwithUltravioletphotometer.UsingahighperformanceThinlayerchromatography,controlledrelativehumidityof67%,alkaloidswithchloroform-methanol-ethylacetate(10:1:0.2)asthedevelopingagent,5%Ninhydrinsolution,theflavonesweredevelopedwithtoluene-chloroform-ethylacetate-aceticacid(7:1:1.8:0.2)asthedevelopingagentand5%aluminiumtrichlorideethanolsolution.ThesewereexaminedundervisiblelightandthethinlayerchromatogramswereanalyzedbyScanner.RESULTSTakingWogoninasthestandard,thecontentoftotalflavonoidswasdeterminedintherangeof4.20mg/g~14.95mg/g.theTLCwascomposedof3characteristicspots,andtheRFvalueofWogoninwasabout0.46.TakingEvolitrineasthestandard,athinlayerchromatographywasestablished,whichwascomposedof7characteristicspots,andtheRfvalueofEvolitrinewasabout0.67.ThequalityofdifferentbatchesofTrigeminalbitterness