海绵来源真菌 Aspergillus sp. 次级代谢产物及其抗菌活性研究.docVIP

  海绵来源真菌 Aspergillus sp. 次级代谢产 物及其抗菌活性研究# 孙玲玲1,2，邵长伦1，邢倩1，王长云1** 5 10 15 20 25 30 35 （1. 中国海洋大学医药学院，海洋药物教育部重点实验室，山东 青岛 266003； 2. 中国科学院海洋研究所，分析测试中心，山东 青岛 266071） 摘要：目的 对一株分离自海绵 Xestospongia testudinaria 的真菌 Aspergillus sp. 的次级代谢 产物及其抗菌活性进行研究。方法 采用溶剂萃取、正反相硅胶柱层析、Sephadex LH-20 凝 胶柱层析及制备 HPLC 等方法，对菌株发酵产物进行提取分离纯化，通过理化性质及波谱 学手段进行化学结构鉴定，以试管连续稀释法评价化合物的抗菌活性。结果 从该菌发酵产 物中分离获得 5 个甲苯酚三聚体类化合物 (1?5) 和 2 个没药烷倍半萜类化合物 (6, 7)，分 别为 expansol B (1)、expansol C (2)、expansol D (3)、expansol E (4)、expansol F (5)、(+)-sydonic acid (6) 和 (+)-sydowic acid (7)。抗菌活性评价表明，化合物 1、2 和 4 表现了中等强度的 抗菌活性。 关键词：海绵来源真菌；Aspergillus sp.；次级代谢产物；抗菌活性 中图分类号：R9 Secondary metabolites and their antibacterial activity from a sponge-derived fungus Aspergillus sp. SUN Lingling1,2, SHAO Changlun1, XING Qian1, WANG Changyun1 (1. Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy,Ocean University of China, ShanDong QingDao 266003; 2. Analytical and Testing Center, Institute of Oceanology, Chinese Academy of Sciences, ShanDong QingDao 266071) Abstract: Objective To investigate the secondary metabolites and their antibacterial bioacty of a fungus Aspergillus sp. isolated from the sponge Xestospongia testudinaria. Methods The extract of the fungal fermentation broth was isolated and purified by solvent extraction, column chromatography on Silica gel, ODS and Sephadex LH-20, and preparative HPLC. Their structures were established by physicochemical properties and spectroscopic analysis. The antibacterial activity was evaluated by the conventional broth dilution assay. Results Seven compounds were identified from the fungal fermentation broth, including five polyphenols containing both phenolic bisabolane and diphenyl ether units, expansols B-F (1?5), and two bisabolane sesquiterpenoids, (+)-sydonic acid (6) and (+)-sydowic acid (7). Compounds 1, 2 and 4 exhibited moderate antibacterial activity. Keywords: sponge-derived fungus; Aspergillus sp.; secondary metabolite; antibacterial activi