松嫩草地增温和氮沉降对丛枝菌根真菌孢子多样性的影响.docVIP

  松嫩草地增温和氮沉降对丛枝菌根真菌孢 子多样性的影响# 巴雷1，武玉涛2* 5 10 15 20 25 30 35 40 （1. 东北师范大学草地科学研究所，植被科学教育部重点实验室，长春 130024； 2. 东北师范大学生命科学学院，长春 130024） 摘要：丛枝菌根真菌是陆地生态系统中重要组成部分，能够与约 80%的高等植物形成丛枝 菌根。它不仅能够提高植物的适应性，而且在维持生态系统物质循环，生长力、多样性和稳 定性方面具有重要功能。本文主要采用原位控制实验方法，研究了松嫩草地模拟增温与氮沉 降对土壤 AM 真菌孢子多样性的影响。结果表明：实验中共鉴定出 26 种的 AM 真菌孢子， 分别隶属于球囊霉属、无梗囊霉属和巨孢囊霉属；球囊霉属的 AM 真菌的种类和数目最多， 其中地球囊霉、膨果球囊霉和幼套球囊霉为优势种；增温与氮沉降对 AM 真菌孢子的种类 组成和密度存在显著影响，并且提高了孢子的丰富度，但是对 AM 真菌孢子的多样性指数 与均匀度指数没有显著影响。 关键词：松嫩草地；丛枝菌根真菌；孢子多样性；增温；氮沉降 中图分类号：Q938 Effects of warming and nitrogen deposition on the diversity of arbuscular mychorrizal fungi in the Songnen Grassland Ba Lei1, Wu Yutao2 (1. Institute of Grassland Science, Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, Northeast Normal University, ChangChun 130024; 2. School of Life Science, Northeast Normal University, ChangChun 130024) Abstract: Abuscular mychorrizal fungi (AMF) are important components of terrestrial ecosystem because of their strong association with more than 80% plants. AMF could not only improve the fitness of plants, but also play an important role in maintaining the nutrient cycling, productivity, diversity and stability of ecosystem. To study the effects of stimulating warming and nitrogen deposition on the diversity of AMF in the Songnen Plain, a manipulated field experiment was carried out for three years. Results showed that there were twenty-six AMF were identified in the soil samples, belonging to Glomus, Acaulospora and Gigaspora. The AMF species in Glomus were abundant and Glomus geosporum, G. pansihalos and G. etunicatum were the dominant species. Warming and adding nitrogen have significant effects on the composition and density of AMF spore, and also improve the richness of AMF community. However, shannon-wiener index and evenness index of AMF comunity were not influenced by warming and nitrogen deposition. Keywords: Songnen Plain; arbuscular mychorrizal fungi; diversity; warming; nitrogen deposition 0 引言