柚皮苷对烟熏加脂多糖诱导的大鼠慢性阻塞性肺病的抑制作用.docVIP

  柚皮苷对烟熏加脂多糖诱导的大鼠慢性阻 塞性肺病的抑制作用# 聂怡初，李沛波，龙康，苏薇薇** 5 10 15 20 25 30 35 40 45 （中山大学，生命科学学院，广州，510275） 摘要：目的：研究柚皮苷对烟熏加脂多糖诱导的大鼠慢性阻塞性肺病(COPD) 的抑制作用。 方法：SD 大鼠 70 只，随机分为空白对照组、烟熏+脂多糖模型组、地塞米松组、氨溴索组、 柚皮苷低剂量组、柚皮苷中剂量组、柚皮苷高剂量组，每组 10 只。除空白对照组外，其余 大鼠造模第 1 天经气道滴注脂多糖(200 μg/kg，20 μg/ml)，从第 2 天开始，连续 8 周每天 早晚置于烟熏箱内连续烟熏各 1 h。各给药组在每天早晨烟熏前 1 h 灌胃给予各受试药物。 造模结束后，各组大鼠经心脏放血处死，手术剖开胸腔后，结扎右侧肺组织，并对左侧肺组 织进行支气管肺泡灌洗，检测肺泡灌洗液中炎性细胞浸润、肿瘤坏死因子 (tumor necrosis factor，TNF-α)、白介素-8 (interleukin-8，IL-8)以及 MUC5AC 粘蛋白分泌的变化，肺组织 中 性 粒 细 胞 髓 过 氧 化 物 酶 (myeloperoxidase ， MPO) 和 金 属 基 质 蛋 白 酶 (matrix metalloproteinase，MMP-9)的含量，并采用 HE 染色与 PAS 染色观察肺组织的病理变化。结 果：柚皮苷可剂量依赖性地显著抑制烟熏加脂多糖诱导的炎性细胞浸润、TNF-α、IL-8 与 MUC5AC 的分泌，以及肺组织中 MPO 与 MMP-9 的含量；并可剂量依赖性地改善模型大鼠 肺泡扩张、气道增厚及杯状细胞增生等病理变化。结论：柚皮苷对烟熏加脂多糖诱导的大鼠 慢性阻塞性肺病模型中炎症、粘蛋白高分泌以及肺气肿均具有明显抑制。 关键词：慢性阻塞性肺病；柚皮苷；烟熏；脂多糖；大鼠；慢性炎症 中图分类号：R285.5 Effect of Naringin on Rat Model of Chronic Obstructive Pulmonary Disease Induced by Cigarette Smoke Plus Lipopolysaccharide Nie Yichu, Li Peibo, Long Kang, Su weiwei (School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275) Abstract: Objective: To observe the potential protective effects of naringin in cigarette smoke plus lipopolysaccharide (CS+LPS)-induced chronic obstructive pulmonary diseases (COPD) rats. Methods: Seventy Sprague-Dawley rats were randomly divided into seven groups: normal control, CS+LPS alone, dexamethasone group, ambroxol group, and three naringin groups. Rats in normal control group were instilled intratracheally with saline, while others were instilled intratracheally with LPS (200 μg/kg，20 μg/ml) on the first day. Then, all LPS pretreated rats were modeled by repeating exposure to CS for 8-wk, 1 h after dealt with placebos, dexamethasone, ambroxol, or naringin, respectively. At the end of the modeling, rats were sacrificed by heart bloodletting using vacuum tubes from the left side of heart, and the left lung lobes were ligated, while the right lung lobes were lavaged. Then, the influx of inflammatory cells, secret