内源性硫化氢通过肠神经系统抑制胃的运动.docVIP

  Inhibition of gastric motility by endogenous H2S via enteric nervous system#  5 10 15 20 25 30 35 40 45 YE Yanfang, LU Wen, ZHU Jianchun, DU Yahui, LIU Kejing, QIN Junfang, FENG Mei, LUO Yan, LIU Chuanyong** (Department of Physiology Shandong University School of Medicine, JiNan 250012) Abstract: Hydrogen Sulfide (H2S) might be a gastrotransmitter in enteric nervous system and might be involved in the regulation of gastrointestinal motility and secretion. This study was conducted to investigate the effect of endogenous H2S on gastric motility. Methods. In in vivo experiments, the intragastric pressure of rats were monitored, and in in vitro experiments, the spontaneous contraction of muscle strips of gastric body was recorded. The location of cystathionine-beta-synthetase (CSE) and cystathionine-gama-lyase (CBS) in stomach was studied by immnohistochemistry staining and Western blot. Key Results: Systemic L-cysteine (100 μmol /kg，i.p.) significantly decreased intragastric pressure. In vitro, L-cysteine (10-4 M–5×10-3 M) and S-adenosyl-l-methionine (SAM) (5×10-4 M–5 ×10-3 M) significantly decreased the tension of the muscle strips from gastric body. This effect of L-cysteine on muscle strip was not influenced by pretreatment of L-NAME and glibenclamide but was reversed by TTX. In in vitro experiments, administration of PAG (10-6 M-10-3 M), the specific inhibitor of CSE, or AOAA (10-6 M), the specific inhibitor of CBS, significantly increased the mean tension of gastric muscle strips. Both PAG and AOAA significantly reversed the inhibitory effect of L-cysteine on gastric motility in vitro. Both CBS and CSE were located in the myenteric plexus of stomach. Conclusions Inferences : We concluded that endogenous H2S inhibited gastric motility via ENS. H2S might be a physiologic relaxant of gastric smooth muscle and exert tonic inhibition on gastric motility. Keywords: Hydrogen sulfide; stomach; smooth muscle; gastric motility; L-cysteine 0 Introduction Gaseous tran