mrflbA缺失突变株液态发酵过程中蛋白酶和几丁质酶活力的变化研究.docVIP

  mrflbA 缺失突变株液态发酵过程中蛋白酶 和几丁质酶活力的变化研究# 杨依珊1，邵彦春2，陈福生2** 5 10 15 20 25 30 （1. 华中农业大学食品科技学院，武汉 430070； 2. 华中农业大学食品科技学院， 武汉 430070） 摘要：为了解红曲菌 G-蛋白信号调节子 mrflbA 缺失突变株的生理、生化特征，测定了该突 变菌株的生物量、葡萄糖利用率、蛋白酶和几丁质酶的活性。与出发菌株红色红曲菌相比较， mrflbA 缺失突变株在第 7 天即比出发菌株提前 2 天进入迅速衰亡期。在发酵过程中葡萄糖 消耗率、蛋白酶和几丁质酶均高于出发菌株，其中蛋白酶活力在第 6 天达到最高，约为出发 菌株的 3 倍，这些结果初步表明 mrflbA 缺失突变株在整个生长过程中具有自溶的显著特征， 推测高活性的蛋白酶是诱发菌体自溶的主要水解酶。研究结果为进一步理解 mrflbA 介导的 红曲菌自溶机理奠定基础。 关键词：微生物；红色红曲菌；G-蛋白信号调节子；水解酶 中图分类号：Q939.97 study on the activities of protease and chitinase of the mrflbA-deleted mutant of Monascus ruber in the submerged fermentaion Yang Yishan, Shao Yanchun, Chen Fusheng (College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070) Abstract: In this paper, conventional analysis including biomass decline, glucose consumption rate and the activities of protease/chitinase were investigated for understanding the physiological and biochemical characteristics during the submerged fermentation of mrflbA deletion mutant of Monascus ruber M-7. Compared with the original strain M-7, the mutant rapidly entered the declining stage at the 7th day, which was earlier than that of M-7 by 2 days. During the whole fermentation, glucose consumption rate and the activities of protease/chitinase were all higher than those of M-7. Obviously, the higest activity of protease occurred at the 6th day, which was approximately 3 times as high as that of the M-7. All these results showed that mrflbA deletion mutant was of typical autolysis characteristics in the growth prossess, and autolysis of mrflbA deletion mutant may be attributed to high protease activity. This study laid a foundation for further understanding of the autolysis mechanism of Monascus spp.. Key words: microorganism; Monascus ruber; regulator of G-protein signaling ; hydrolyases  35  0  引言 G 蛋白信号调节子(Regulators of G-Protein Signaling, RGSs)是一类与激活的 GTP-G 蛋白 α 亚基相互作用，从而调节 G 蛋白信号强度和持续时间的蛋白质[1]。对多种丝状真菌 G 蛋 白信号调节子的功能研究表明，它们在调控生长发育、次生代谢物分泌以及致病性生命活动 中发挥着重要