pH调控的两性多肽表面活性剂自组装.docVIP

  pH 调控的两性多肽表面活性剂自组装# 曹美文，曹长海* （中国石油大学（华东）生物工程与技术中心，山东 青岛 266555） 5 10 15 20 25 30 35 40 摘要：综合运用圆二色光谱、透射电镜、原子力显微镜以及 Zeta 电位测定等多种技术手段， 研究了两性多肽表面活性剂分子 I3CGK-OH 的 pH 响应性自组装行为。I3CGK-OH 分子含有三个 pH 响应性基团，分别是羧基、巯基和伯氨基，因而随 pH 变化可以带有不同的电荷。所研究 的三个 pH 值 2.5、7.0、12.0 下 I3CGK-OH 分子所带净电荷分别为+0.5、0、–1.9。净电荷 性质和数量的不同导致分子组装过程和分子构象的差异。pH 值为 7.0 时，分子带电量接近 0， 分子间静电斥力很弱，分子可以在很低的浓度就完成组装平衡；分子组装时采取紧密折叠堆 积的构象，所形成的初级纤维直径较小；纤维表面带电量也较低，导致纤维有很强的侧向堆 积作用，形成条带状高级结构。而在 pH 值 2.5 和 12.0，分子带有较多电荷，分子间静电斥 力增大，分子的组装平衡浓度也相应增加；分子组装时采取较为伸展的构象，所形成的初级 纤维直径较大；纤维表面带电量也较高，纤维可以单独存在，也可以两根或多根纤维互相扭 结形成高级组装体。本研究阐明了 pH 值影响多肽表面活性剂组装行为的机理，对探索可控 的自组装体构筑方法有很强的指导意义。 关键词：多肽表面活性剂；自组装；纳米纤维 中图分类号：O648 pH-tuned self-assembly of zwitterionic peptide surfactant CAO Meiwen, CAO Changhai (Center for Bioengeering and Biotechnology, China University of Petroleum (East China), ShanDong QingDao 266555) Abstract: In this paper, the pH-responsive self-assembly of a zwitterionic peptide surfactant, I3CGK-OH, was studied using a combination of techniques including circular dichroism, transmission electron microscopy, atomic force microscopy, and zeta potential measurements. I3CGK-OH has three pH-responsive groups, which are a carboxyl group, a hydrosulfide group, and a primary amine group. The molecule can bear different charges with changing of pH value. At pH 2.5, 7.0, and 12.0 the molecule is charged as +0.5, 0, and –1.9, respectively. Such a difference in charge affected the self-assembly process as well as the molecular conformation in the assembly. At pH 7.0, I3CGK-OH is nearly zero charged, and low inter-molecular charge repulsion was the case. Hence it could fufill the self-assembly process at relatively lower concentration. The molecule took a compact conformation during assembly and the formed primary fibers gave smaller diameters. These primary fibers were also lower-charged on their surface and they exhibited high propensity for lateral association and formed sheet-like higher-order structures by parallel aligning. While at