0502 动物细胞融合与单克隆抗体.pptVIP

Mono: One Clone: A strain of cells descended form a single cell Antibody: A molecule of animal origin that has immunological activity only against the antigen to which it was made Hybridoma cells fusion of lymphocytes (normal blood cells for antibody production) with myeloma (cancer) cells How to make a monoclonal antibody? Immunize mice Test the serum Perform a fusion Screen the fusion for the right cells Grow the hybridomas Harvest the antibody Concentrate and purify the product Summary 细胞融合的定义、原理与方法 融合细胞的筛选方法：杂交瘤细胞的筛选方法 单克隆抗体的制备方法 Want to know more? Just ask! 洗脱液 支持物 被分离物质 被分离物的抗体 其它物质 亲合