正交试验设计优化茶子木层孔菌总多糖的提取条件.pdfVIP

20 食品与药品 Food and Drug 2009 年第 11卷第09期 正交试验设计优化茶 子木层孔菌总多糖的提取条件 藨 1，2 1* 刘玉红 ，王凤山 （1．山东大学药学院，山东 济南 250012；2. 山东中医药大学药学院，山东 济南 250355） 摘 要：目的 研究提取茶藨子木层孔菌总多糖的最佳条件。方法 用蒽酮-硫酸法测定总多糖含量，以提取温度、时间、 次数、溶剂用量等4项因素，设计3个水平进行正交试验。结果 最佳工艺为15倍量水，90 ℃提取4 h ，共3次。结论 本实验 确定的最佳条件稳定性好且简便易行。 关键词：茶藨子木层孔菌；多糖；正交试验 中图分类号：Q539 文献标识码：A 文章编号：1672-979X （2009 ）09-0020-03 Optimization of Extraction Conditions of Total Polysaccharides from Phellinus ribis by Orthogonal Design LIU Yu-hong1,2, WANG Feng-shan1 (1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China; 2. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China) Abstract: Objective To study the optimal extraction conditions of the total polysaccharides from Phellinus ribis . Methods The total polysaccharides content was determined by the anthrone-sulfuric acid method. The extraction 4 temperature, extraction time, extraction times and solvent volume were involved in a L (3 ) orthogonal experiment. 9 Results The optimal extraction conditions were hot water (w/v) of 1:15, extraction at 90 ℃ for 4 h, three times repeated. Conclusion With good stability and convenient processing, the method in this experiment is feasible for the extraction of the total polysaccharides from Phellinus ribis . Key Words: Phellinus ribis ; polysaccharide; orthogonal test Phellinus ribis 茶藨子木层孔菌 （ ）为锈革孔菌科 医药大学生药教研室徐凌川教授鉴定。葡聚糖4万 （中 Hymenochaetaceae Phellin