马兰籽油中脂肪酸的不同甲酯化方法与GC-MS分析.pdfVIP

※分析检测 食品科学 2009, Vol. 30, No. 22 253 姜显光，侯冬岩，回瑞华，李铁纯，刘晓媛，李秀铭 (鞍山师范学院化学系，辽宁鞍山 114007) 采用索氏提取法对马兰籽中马兰籽油进行提取，分别采用两种方法进行甲酯化处理，以气相色谱- 质谱联 用仪进行分析，对它们的脂肪酸组成和相对含量进行比较。结果表明：酯化方法l 和酯化方法2 分别鉴定出16 种 和6 种脂肪酸，占马兰籽油总量的98.66% 和63.68%，两种方法酯化的马兰籽油中鉴定出主要脂肪酸均为十八碳二 烯酸、十八碳烯酸、十六酸。 马兰籽；脂肪酸；索氏提取法；酯化方法；气相色谱- 质谱联用仪 Different Esterification Method for Analysis of Fatty Acids in Kalimeris indica Seed Oil by GC-MS JIANG Xian-guang ，HOU Dong-yan ，HUI Rui-hua ，LI Tie-chun ，LIU Xiao-yuan ，LI Xiu-ming (Department of Chemistry, Anshan Normal University, Anshan 114007, China) Abstract ：Kalimeris indica seed oi1 was extracted by Soxhlet extraction. The fatty acids in Kalimeris indica seed oil were esterified by two methods and analyzed by gas chromatography-mass spectrometry (GC-MS).The components and relative contents of the fatty acids esterified by two methods were compared. The results showed that 16 fatty acids were identified by the first esterification method and 6 fatty acids were identified by Esterification Method 2. The contents of the identified fatty acids accounted for 98.66% and 63.68% of Kalimeris indica seed oil, respectively. The predominant fatty acids of Kalimeris indica seed oil were octadecadienoic acid, octadecenoic acid and Hexadecanoic acid by two esterification methods. Key words ：Kalimeris indica seed ；atty acid ；soxhlet extraction ；esterification method ；gas chromatography-mass spectrometry(GC-MS) 中图分类号：O657.63 ；Q949.783.5 文献标识码：A 文章编号：1002-6630(2009)22-0253-03 马兰(Kalimeris indica (L.) Sch.-Bip.)系菊科马兰属植 内报道罕见，对其脂肪酸成分进行分析，可为其开发 物。为多年生直立草本，高30 ～70cm 。分布于全国各 利用提供科学依据。本实验采用索氏提取法对马兰籽油 省区；亚洲南部及东部广布。并富含多种维生素，在 进行提取，分别采用两种方法进行甲酯化处理，用GC- 民间作蔬菜食用。全草入药，能消食积除湿热、利小