不同磁场对大鼠血清MDA含量及GSH-Px活性的影响.docVIP

不同磁场对大鼠血清MDA含量及GSH-Px活性的影响 郭梅凤，张桂莲，苏琨，郑东华 （包头医学院药学院，内蒙古包头，014040） 摘要: 目的 探讨不同磁场对大鼠血清丙二醛(MDA)含量及谷胱甘肽过氧化物酶（GSH-Px）活性的影响。方法 将大鼠置于不同磁场（10 mT、20 mT、 30mT）中每天曝磁30min, 15天后处死大鼠，检测磁场对大鼠血清中MDA含量及GSH-Px活性的影响。TBA显色法测定MDA含量；二硫代二硝基苯甲酸法检测血清中GSH-Px活性。结果 在30mT磁场中曝磁的大鼠血清中MDA含量显著低于对照组（p0.05）；GSH-Px活性显著高于对照组（p0.01），其它场强MDA含量和GSH-Px活性与对照组相比无统计学意义。 结论 一定强度的磁场对大鼠血清的脂质过氧化代谢产生影响，降低了过氧化物的生成。 关键词: 磁场；谷胱甘肽过氧化物酶；丙二醛；血清 中图分类号: R-332 R322.61 文献标识码： A 文章编号 Effects of the different magnetic field on the big rat serum MDA contents and GSH-Px activity GUO Mei- feng, ZhANG Gui -lian, SU Kun, ZhENG Dong -hua (the medicine college in BaoTou medical college, Inner Mongolia, BaoTou, 014010) ABSTRACT： Objective the impact of the different magnetic field upon the big rat serum malondialdehyde (MDA )contents and glutathione-peroxidase( GSH-Px )activity. Methods Place big rat at the exposed to the different magnetic field (10 mT, 20 mT and 30 mT) in Everyday 30 minutes，The big rats were killed 15 days later and examines magnetic fields impact for the MDA contents and GSH-Px activity in the big rat serum.TBA shows the color method measuring MDA contents; Dithio -dinitrobenzoic acid method determineds GSH-Px in the serum activity Results after they had been exposed to 30 mT magnetic field , MDA contents in the big rat serum is lower than control group significantly;(p0.05)； the-activity of GSH-Px is higher than control group significantly (p0.01), other magnetic field intensity MDA contentses and GSH-Px activity and the matched control to compare to have no statistics meaning in the big rat serum.Conclusions The magnetic field of certain intensity generates impact on metabolism of lipoperoxidation of the big rat serum, Lowered peroxide of creation Keyword: Magnetic field; glutathione-peroxidase ; malondialdehyde ； Serum Chinese Library Classification:R-332, R322.61 Document code?: A Article ID: 前言 磁场在多方面对生物体有明显的生物效应，磁场具提高机体抗氧化酶活性的。MDA含量及GSH-Px活性的影响，旨在进一步了解磁场对生物机体自由基代谢的作用，