微小RNA 31通过 RhoBTB1调控人肺腺癌侧群细胞对顺铂敏感性的研究.docVIP

  微小 RNA 31 通过 RhoBTB1 调控人肺腺癌 侧群细胞对顺铂敏感性的研究# 孙婧，束永前** 5 10 15 20 25 30 （南京医科大学第一附属医院肿瘤科，南京，江苏，210029） 摘要：目的：探讨 miR 31 是否可调控人肺腺癌侧群（SP）细胞对顺铂敏感性及其相关途径； 方法：利用 Hoechst33342 染料法，通过流式细胞仪紫外激光分选功能分选出肺癌 A549 细胞 中的 SP 细胞；使用 Liptamine 2000 系统将 hsR-miR-31、miR 31 siRNA 等转入 SP 细胞， real-time PCR 检测 miR 31 在 SP 细胞内的表达，Western-blot 检测 RhoBTB1 的表达变化； MTS 法检测转染后顺铂处理的 SP 细胞吸光值。结果:通过转染，miR 31 表达水平可成功下 调或上调；SP 细胞中 miR 31 的表达可影响 RhoBTB1 的表达水平；miR 31 的表达可影响 SP 细胞对顺铂的敏感性，此作用可由 RhoBTB1 水平改变而部分拮抗。结论：miR 31 可通过 RhoBTB1 调控人肺腺癌 SP 细胞对顺铂的敏感性，为研究肿瘤耐药提供新的分子机制和靶 点。 关键词：肺癌，微小 mRNA 31，鸟苷酸三磷酸酶 1，侧群细胞，顺铂 miRNA 31 regulates the sensitivity of human lung cancer side population (SP) cells to cisplatin through RhoBTB1. Sun Jing, Shu Yongqian (Department of Medical Oncology, The First Affiliated Hospital of Nanjing Medical University,Nanjing, Jiangsu Province, 210029) Abstract: Objective: To investigate whether miR 31 can regulate the sensitivity of human lung adenocarcinoma side population (SP) cells to cisplatin and related pathway ; Methods:SP cells were isolated using flow cytometry and Hoechst 33342 dye efflux assay from human lung adenocarcinoma A549 cells. hsR-miR 31, miR 31 siRNA were transfected into SP cells using Liptamine 2000 systems and the expression of miR 31 was measured by real-time PCR. The expression of RhoBTB1 was tested by Western lot. MTS test was used to observe the growth differences between cells after transfection and cisplatin treatment. Results: The expression of miR 31 can be successfully upregulated or downregulated by transfection; the expression of RhoBTB1 can be modulated miR 31. The sensitivity of SP cells to cisplatin can be affected by miR 31 which can be rescued by RhoBTB1 partly. Conclusion: miR 31 regulates the sensitivity of human lung cancer side population (SP) cells to cisplatin through RhoBTB1. It should be a new mechanism and target for the cancer research of drug resistance. Key words: lung cancer; micro RNA 31; RhoBTB1; Side population cells (SP cells); ci