响应面法优化（R）-α-羟基苯乙酸培养基.docVIP

  响应面法优化（R）-α-羟基苯乙酸培养基# 陈秉梅，许小平** （福州大学化学化工学院，福建，福州，350116） 5 10 摘要：目的: 采用响应面分析法对菌株 Bacillus sp.ULi-11 发酵产（R）-α-羟基苯乙酸培养 基进行优化。方法: 采用 Plackett-Burman 试验设计筛选出影响发酵产率的三个主要因素，采 用最陡爬坡路径逼近最大响应区域, 再用 Box-Behnken 试验设计及响应面分析法进行回归 分析。结果: 最佳培养基浓度分别为：酪蛋白胨 12.81g/L，淀粉 12.74g/L，KH2PO4 2.97g/L， K2HPO4 1.2 g/L，MgSO4 0.15 g/L，CaCl2 0.125 g/L，MnSO4 0.015 g/L，ZnSO4 0.01 g/L。 在优化条件下，（R）-α-羟基苯乙酸产率达 69.78%，比优化前提高了 37.6%。结论: 响应 面分析法用于寻求（R）-α-羟基苯乙酸发酵的最佳培养基组分浓度是可行的。 关键词：（R）-α-羟基苯乙酸；Plackett-Burman；响应面分析法；培养基优化；Bacillus sp. 中图分类号：TQ015.9 15 Optimization of (R)-α-Hydroxyphenylacetic Acid Fermentation Medium by Using Response Surface Methodology CHEN Bingmei, XU Xiaoping 20 25 30 35 40 (College of Chemistry and Chemical Engineeing, Fuzhou University, Fujian 350116，China) Abstract: Purpose: Response Surface Methodology was applied to optimize the culture components for (R)-α-hydroxyphenylacetic acid by Bacillus sp.ULi-11. Methods: By the initial screening method, Plackett-Burman design was used to evaluate the influence of total eight related factors. It showed that three factors playing the important roles in the medium. Path of steepest ascent was undertaken to approach the optimal region of the fermentation conditions subsequently. Finally, the concentrations of those three main factors were further optimized by using Box-Behnken and Response Surface Analysis. Results: By solving the quadratic regression model equation, the optimal concentrations of the variables were determined as: starch 12.81g/L, casein peptone 12.74g/L, KH2PO4 2.97g/L, K2HPO4 1.2 g/L, MgSO4 0.15 g/L, CaCl2 0.125 g/L, MnSO4 0.015 g/L, ZnSO4 0.01 g/L. The yield of (R)-α-hydroxyphenylacetic acid (69.78%) was promoted by 37.6% at the optimal culture conditions, compared to that obtained from initial fermentation conditions. Conclusion: It is available to find the optimal medium for (R)-α-hydroxyphenylacetic acid by response surface methodology. Key words: (R)-α-Hydroxyphenylacetic Acid, Plackett-Burman, Response Surfa