人类端粒酶基因反义核酸对恶性肿瘤细胞端粒酶抑制的实验研究.pdfVIP

第 12 卷　第 1 期 河南医学研究 Vol. 12 　 No. 1 2003 年　　 3 月 HENAN 　MEDICAL 　RESEARCH March 　　2003 ( ) 文章编号 :1004437X 2003 ·基础研究· 人类端粒酶基因反义核酸对恶性肿瘤细胞端粒酶 抑制的实验研究 胡杰英 , 蒋东霞 , 何 　琳 (河南省肿瘤研究所医学生物室 　河南郑州 　450003) ( ) 摘要 : 目的: 研究人类端粒酶 hTR 基因的反义核酸对 K562 细胞、Hep2 细胞端粒酶活性和 K562 细胞凋亡 的影响。方法 : 采用 TRAP 法检测 K562 细胞、Hep2 细胞在反义核酸处理前后端粒酶活性的变化以及采用 TUNEL 法观察反义核酸处理前后 K562 细胞凋亡的变化。结果 : hTR 基因反义核酸能有效抑制 K562 细胞和 Hep2 细胞的端粒酶活性 ,并且诱导 K562 细胞发生凋亡。结论 : hTR 基因反义核酸能显著抑制肿瘤细胞的 端粒酶活性 ,为恶性肿瘤治疗提供了一条新的有效的途径。 关键词 : 端粒酶 ; 细胞 ; 反义治疗 ; 凋亡 中图分类号: R99 　　文献标识码 : A Study of antisense oligonucleotide against hTR RNA on the telomerase activity in K562 and Hep2 cells and apoptosis in K562 cells HU Jieying , J IANG Dongxia , HE Lin ( ) Henan Tumor Institute , Zhengzhou 450003 , China Abstract : Objective : To study the inhibitory effect of human telomerase activity in K562 and Hep2 ( ) cells and the apoptosis by an antisense oligonucleotide against human telomerase RNA hTR in K562 cells. Methods : Telomerase activity of K562 and Hep2 cells with antisense treatment was measured us ing the TRAP assay and apoptosis was studied by means of TUNEL method. Results : The telomerase ac tivity was effectively inhibited and the apoptosis was induced by the antisense treatment. Conclusion : Antisense oligonucleotide against hTR can effectively inhibit the telomerase activity of tumor cells ,and subject is an effective and novel approach for the treatment of malignant cancers. Keywords : telomerase ; cell ; antisense treatme