虫类药复方干预大鼠肺间质纤维化进程的实验研究.docVIP

  虫类药复方干预大鼠肺间质纤维化进程的 实验研究# 吴华阳，苏惠萍，张立山，闫玉琴* 5 10 15 20 25 30 35 （北京中医药大学东直门医院呼吸科，北京 100700） 摘要：目的:观察不同时相应用虫类药复方，对博莱霉素致大鼠肺间质纤维化的影响，研究 虫类药复方所代表的搜剔肺络法在不同时相对肺间质纤维化的干预作用，为指导临床用药选 择最佳治疗时机。方法: 128 只 SD 大鼠随机分为假手术组、模型组、中药组、西药激素组， 每组 32 只；除假手术组外，其余各组一次性气管内滴注博莱霉素制备肺纤维化大鼠模型， 假手术组气管内注射等量生理盐水，中药、西药组于造模后 24 小时开始给予相应的干预药 物，假手术、模型组给予生理盐水，分别于第 7 天、14 天、28 天、45 天处死，取肺组织行 HE、Masson 染色，并比较不同时相肺组织病理改变。结果: 与模型组比较，虫类药复方治 疗组各时相肺泡炎和肺纤维化程度明显减轻，其中第 7d、45d 时作用更明显，具有统计学差 异（P＜0.05）。结论: 虫类药复方对博莱霉素 A5 诱导的大鼠肺纤维化有一定的抑制作用, 提 示早期及长期用药效果更好。 关键词：中医内科学；虫类药复方；肺间质纤维化 中图分类号：R21 Experimental Study on Effect of Drug Prepared from Insects Formula in Treating Pulmonary Fibrosis in Rat at Various Time Phases WU Huayang, SU Huiping, ZHANG Lishan, YAN Yuqin (Respiratory Department of Dongzhimen Hospital,Beijing University of Chinese Medicine, Beijing 100700) Abstract: Objective:To observe the preventive effects of drug prepared from insects formula on blemycin-induced pulmonary interstitial fibrosis in rats．Method：One hundred and twenty-eight SD rats were divided into control，model，hydrocortisone and drug prepared from insects formula group randomly.The rats in model group and the treatment groups were injested by blemycin A5 within the trachea to establish pulmonary fibrosis model，while the same volume of normal saline in the sham-operation group. Treatments were started after 24hours of the model establishment with corresponding drug，and oral saline in control group and model group，respectively. Rats were killed on 7/14/28/45 days later，lungs were evaluated by pathological slides with staining of hematoxylin-Masson. Results: Compared with the model group，drug prepared from insects formula can effectively reduce the degree of inflammation and fibrosis, which were obviously improved on 7th and 45th day. Conclution: drug prepared from insects formula has certain treatment effect for pulmonary fibrosis in rats induced by bleomycin A5, the earlier and longer used the fomula, the better effect could be got. Keywo