bFGFCHAARBM生物复合材料生物相容性及成骨效应的实验研究.docVIP

bFGF/CHA/ARBM生物复合材料生物相容性及成骨效应的实验研究 宋扬1* 杨彦君1* 程维1* 1* 北华大学附属医院骨外二科 吉林省吉林市 132400 摘要 目的：探讨碱性成纤维细胞生长因子（bFGF）复合珊瑚羟基磷灰石（CHA）及自体红骨髓（ARBM）修复骨节段性骨缺损中的作用，为进一步临床应用提供理论依据。方法：实验于2009-05/2010-04在北华大学生命科学研究中心完成。取60只家兔制作双侧桡骨干完全缺损1.0cm模型，随机分成4组，每组15只：A（bFGF/CHA/ARBM）组、B（CHA）组、C（自体骨）组，D（旷置）组，于2、4、8、12周进行大体观察，影像学及组织学评估评价各组修复骨缺损的效果。 结果：58只兔子进入结果分析，B组及D组各死亡1只。bFGF/CHA/ARBM组影像学及组织学观察优于单纯CHA组，bFGF/CHA/ARBM组与自体骨组12周时组织学评价无统计学差异。结论：bFGF/CHA/ARBM生物复合材料具有较强的成骨能力，可代替自体骨，成为新型的人工骨。 关键词：碱性成纤维细胞生长因子; 珊瑚羟基磷灰石;自体红骨髓;骨缺损;人工骨;兔子;X线检测;病理组织学观察。 BFGF/CHA/ARBM biological composite material biocompatibility and health effect of experimental research Song Yang1* Yang Yan Jun1* Cheng Wei1* North China university affiliated hospital bone outside 2th families abstract Objective: To explore basic fibroblast growth factor (bFGF) composite coral hydroxyapatite (CHA) and autologous red mulberry (ARBM) to repair of joints segment bone defect of the role, for further provide theoretical basis for clinical application. Methods: The experiment in 2009-05/2010-04 In the north China university life science research center finish.Take 60 rabbits production double side radial backbone completely defect 1.0 cm model, random divided into 4th groups. each group only 15 .A (bFGF/CHA/ARBM) group, B (CHA) group, C(autologous bone) group, D (desert buy) group. In 2, 4, 8, 12 weeks for gross observation Imaging and histological evaluation group to repair bone defect effect. Results: 58 rabbit into the result analysis B and D group of each death one. BFGF/CHA/ARBM group imaging and histological observation group is better than that of pure CHA,BFGF/CHA/ARBM group and autologous bone group of 12 weeks was not statistically different histologic evaluation. Conclusion: BFGF/CHA/ARBM biological composite material has strong osteogenesis ability, but instead of autologous bone, become a new type of artificial bone. Keywords: Basic fibroblast growthfactor;Coral hydroxyapatite;Autologou