鸡卵细胞提取液有维持和增强细胞活性的关键作用.pdfVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(6): 804–809 http ://www.cj DOI: 10.11844/cj cb.2013.06.004 1 鸡卵细胞提取液有维持和增强细胞活性的关键作用 阮光萍 王金祥 庞荣清 姚 翔 阮光洪 朱光旭 朱向情 蔡学敏 何 洁 赵 晶 李自安 石琳琳 王桂华 潘兴华* (解放军昆明总医院干细胞工程实验室, 昆明650032) 摘要 该文鉴定了一种提取物具有维持和增强细胞存活与分化的能力, 这将在细胞生物学 研究中得到应用。制备四种卵细胞提取物, 雄鼠的脾细胞被提取物渗透并培养, 然后输注给雌鼠。 在移植后雌鼠外周血中GFP 阳性细胞和骨髓细胞中的Y染色体被检测，对比脾细胞在体内存活与 分化的不同能力。移植蛋白和全蛋提取液处理的脾细胞的雌鼠外周血检测到GFP 阳性细胞, 在移 植后120 d, 雌鼠骨髓中检测到的Y染色体阳性率与外周血中的GFP 阳性细胞率呈正比。用蛋白和 全蛋提取液渗透脾细胞后, 脾细胞在雌鼠体内存活能力强于其他提取液。结果表明, 鸡蛋白提取液 有维持并增强脾细胞存活的作用。因此蛋白提取物可在将来用于干细胞功能的维持。 关键词 GFP转基因鼠; 脾细胞; 提取物; 细胞存活; 细胞分化 Chicken-egg Extracts Have the Key Roles in Maintaining and Enhancing the Cell Activity Ruan Guangping, Wang Jinxiang, Pang Rongqing, Yao Xiang, Ruan Guanghong, Zhu Guangxu, Zhu Xiangqing, Cai Xuemin, He Jie, Zhao Jing, Li Zian, Shi Linlin, Wang Guihua, Pan Xinghua* (Stem Cell Engineering Laboratory, Kunming General Hospital of PLA, Kunming 650032, China) Abstract This paper concerns the identification of an extract with the ability to maintain and enhance the survival and differentiation of cells which will be useful in cellular biology research. Four types of egg extracts were prepared. The spleen cells from male mice were permeabilized with the extracts and cultured respectively, and then transfused into female mice. The GFP-positive cells in the peripheral blood of female mice and Y chromosome in bone marrow cells were detected to compare the different abilities of spleen cells to survive and differentiate in vivo. Surviving GFP-positive spleen cells were detected in the female mice of chicken-egg-white and whole-egg extracts group. At 120 days after transplantation, the percentage of cells containing a Y