黄瓜中导入野生酸黄瓜外源DNA 片段的分子验证.pdfVIP

中国农业科学 2010,43(22):4663-4670 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.22.013 黄瓜中导入野生酸黄瓜外源DNA片段的分子验证 史建磊，陈劲枫，娄群峰，隋益虎，吴志明，张淑霞 （南京农业大学园艺学院/作物遗传与种质创新国家重点实验室，南京 210095 ） 摘要：【目的】对栽培黄瓜（ Cucumis sativus L.,2n=14）与野生酸黄瓜(Cucumis hystrix Chakr.,2n=24) 经远缘杂交和多代回交、自交而来的渐渗系进行分子标记分析和表型鉴定，旨在为黄瓜导入酸黄瓜外源 DNA 片段 提供相应依据。【方法】从分子标记分析、DNA 序列比对和农艺性状表现上对各渐渗系和双亲进行研究。【结果】 渐渗系与栽培黄瓜存在13.2%的遗传多态性，包括供体特异带、缺失带和产生的新带。利用RAPD引物D-11和SSR 引物 06632 在渐渗系中分别扩增出约 310 bp 和 150 bp 的酸黄瓜特异带，序列分析表明其与酸黄瓜相应 DNA 片段 的相似性分别为 92.93%和 96.48%，碱基差异分别为 41 个和 9 个，包括碱基转换、颠换和缺失。表型鉴定发现其 呈现出酸黄瓜的遗传特性。【结论】野生酸黄瓜DNA已渐渗进入栽培黄瓜基因组，同时发生了少量不同类型的变异。 关键词：黄瓜；野生酸黄瓜；渐渗系；DNA序列；农艺性状 Molecular Verification of DNA Flow from Wild Sour Cucumber to Cultivated Cucumber SHI Jian-lei, CHEN Jin-feng, LOU Qun-feng, SUI Yi-hu, WU Zhi-ming, ZHANG Shu-xia (State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture, Nanjing Agricultural University, Nanjing 210095) Abstract: 【Objective 】 Introgression lines were researched by molecular markers and the phenotypic identification in order to proof DNA flow from wild sour cucumber to cultivated cucumber, which were obtained by interspecific hybridization between the cultivated cucumber (Cucumis sativus L., 2n=14) and wild sour cucumber (Cucumis hystrix Chakr., 2n=24), and multiple generational backcrossing and selfing followed. 【Method 】 Molecular markers, DNA sequences and agronomic traits were used in the study to analyze and contrast introgression lines and their parents. 【Result 】 The results showed that the genetic polymorphism between introgression lines and cultivated cucumber was 13.2%, including donor specific ba