木霉C01产酶优化及木糖渣酶解保护剂研究.pdfVIP

中国科技论文在线 木霉 C01 产酶优化及木糖渣酶解保护剂 研究# 蔡甜甜，张喜宾，潘静，刘钢强，陈世阳，徐海朋，张增明，吴健** 5 （郑州大学生物工程系微生物发酵与代谢工程实验室，郑州 450001） 摘要：在已有研究基础上，通过正交试验对产纤维素酶菌株木霉 C01 的发酵条件进行优化 并选择合适的酶解保护剂以提高产酶活性和水解木糖渣的能力。优化后的培养基为：KH2PO4 0.2%,NaCl 0.01%,MgSO4 0.03%,NaNO3 0.2%,FeCl3 0.001%,CaCl2 0.01%,玉米浆 2%,蛋白胨 0.5%,木糖渣 3%,麸皮 1.5%,玉米芯 1% 。在 250ml 的锥形瓶中进行摇瓶发酵，其中装液量 10 为40ml ，种龄24h ，10%接种量，在28 ℃，180r/min 条件下培养3 天，发酵结束后离心制 得粗酶液, 测定其以木糖渣为底物的酶解活性为54.5U。向酶解液中添加四环素、叠氮化钠、 硫代硫酸钠保护剂可使木糖渣水解效果得到明显提高。 关键词：纤维素酶；发酵；优化培养；保护剂 中图分类号：Q939.97 15 Optimum of fermentation for a cellulase producing strain C01 and its enzyme protective agents CAI Tiantian, ZHANG Xibin, PAN Jing, LIU Gangqiang, CHEN Shiyang, XU Haipeng, ZHANG Zengming, WU Jian 20 (Microbial Fermentation and Metabolic Engineering Laboratory, Bioengineering Department ,ZhengZhou University, ZhengZhou 450001) Abstract: A fungal strain named C01 that is capable of producing cellulases was selected previously in the laboratory. In order to increase its cellulase output by liquid fermentation, orthogonal experiments were used to determine the optimum medium components for the strain. 25 The effect of protective agents on enzyme activity was studied. As the results, the optimum fermentation medium contains KH2PO4 0.2%,NaCl 0.01%,MgSO4 0.03%,NaNO3 0.2%,FeCl3 0.001%,CaCl2 0.01%, corn steep liquor 2%, pepton