纳米粒子组装电化学DNA生物传感器检测Hg2+.pdfVIP

中国科技论文在线 Electrochemical DNA-based Biosensor for the Determination of Mercury (II) Ions Incorporating Gold Nanoparticles Modification# ** 5 LIU Xiaorong, YAN Zhao, LI Yan, ZHENG Jianbin (Northwest University, Xi’an 710069) Abstract: In this paper an electrochemical DNA-based biosensor incorporating a signal enhancement for the determination of mercury (II) ions (Hg2+) in aqueous solution was designed by using a thymine (T)-rich, mercury-specific oligonucleotide (MSO) probe and gold nanoparticles (GNPs) modification. 10 The electrochemical DNA-based biosensors were fabricated by immobilizing the MSO probe onto the surface of GNPs modified gold electrode via thiol-Au interactions. In the presence of Hg2+, the thiolated MSO probe could form stable DNA duplexes by the specific binding interactions between 2+ 2+ 2+ + Hg and thymine (T–Hg –T). The MSO-bound Hg can be electrochemically reduced to Hg , which provides a readout signal for quantitative detection of Hg2+. The reduction peak current is linearly 15 related to the concentration of Hg2+ from 1.0×10-9 M to 1.0×10-6 M with a detection limit of 5×10-10 M. This