丙型肝炎病毒核心抗原的转位表达与肝（癌）细胞凋亡的关系.docVIP

丙型肝炎病毒核心抗原的转位表达与肝（癌）细胞凋亡的关系 【关键词】 ,C型肝炎病毒属 　　关键词: C型肝炎病毒属;癌，肝细胞;蛋白表达 　　摘 要:目的 探讨丙型肝炎病毒核心抗原的转位表达与肝（癌）细胞凋亡的关系及其意义. 方法 采用免疫组织化学方法检测HCV核心区抗原和NS3区抗原在肝硬变（LC）和肝细胞肝癌（HCC）组织中的分布，同时对连续切片进行原位凋亡检测并计算凋亡指数. 结果 HCV核心区抗原定位于肝细胞或肝癌细胞的胞质或胞核中，在LC组织中，核心抗原呈弥漫或灶状分布于肝细胞胞质，偶见胞核阳性的肝细胞，阳性率为60.7%;核心抗原在癌组织中以核阳性分布为主，阳性率为37.6%，癌旁肝组织以胞质阳性为多见.HCC中HCV核心抗原的核阳性率（75.0%，15例/20例）明显高于其在LC（17.7%，6例/34例）及癌旁肝组织（11.8%，2例/17例）中的阳性率（Plt;0.05）;在HCC中，HCV核心抗原核转位表达的癌组织的AI值（0.174±0.093）显著低于胞质阳性的癌组织（0.512±0.113，Plt;0.01）.HCV NS3区抗原定位于肝细胞或肝癌细胞的胞质中，在LC和HCC组织中的阳性率分别为53.6%和39.6%.未发现HCV NS3区抗原的表达与肝（癌）细胞凋亡的相关性. 结论 HCV感染与我国LC，HCC的发生关系密切，HCV核心抗原在肝癌组织中常发生核转位表达，这种核转位表达抑制了肿瘤细胞发生凋亡，从而促进肿瘤组织的进一步恶化. 　　Keywords:hepatitis C-like virus;carcinoma，hepatocellular;protein expression 　　Abstract:AIM To analyse expression of HCV core protein and apoptosis in formalin-fixed paraffin-embedded tissues of liver cirrhosis and hepatocellular carcinoma.METHODS Immunohistochemistry method was used to show the dis-tributive pattern of HCV core and NS3antigen.Modified in situ end labeling（ISEL）was conducted on the serial sec┐tions.RESULTS Immunohistochemistry showed expression of HCV core protein in the liver cirrhosis of34/56cases and HCC of20/53cases.In liver cirrhosis and pericancerous tis-sues，the signals were mainly localized in the cytoplasm of hepatocytes.ISEL showed that only cells expressing core protein might initiated apoptosis.In cancerous tissues，translocated expression of HCV core protein was observed in the nuclei of tumor cells.According to ISEL on serial sec-tions，all these positive tumor cells hardly exhibited apoptosis（AI for nuclear positive，0.174vs AI for cytoplasmic posi-tive，0.152，Plt;0.01）.HCV NS3antigen was mainly dis-tributed in the cytoplasm of hepatocytes.We could hardly found the relationship between the expression of NS3and apoptosis in hepatocellular carcinoma.CONCLUSION HCV core protein may express in the nuclei of tumor cell.Translo-cated expression of HCV core protein may inhibit apoptosis i