基质金属蛋白酶2，9基因多态性与早发冠心病遗传易感性的研究.docVIP

基质金属蛋白酶2，9基因多态性与早发冠心病遗传易感性的研究 作者：张岩，王聪霞，董新，朱参战， 段宗明，党寅虎，魏瑾，韩振华，李永勤，张荣 【摘要】 目的 探讨中国陕西地区汉族人群基质金属蛋白酶2(matrix metalloproteinase2, MMP2)基因rs2285053及基质金属蛋白酶9(MMP9)基因rs3918242单核苷酸多态性与早发冠心病(premature coronary artery disease, PCAD)发病的关联性。方法 应用聚合酶链反应限制性片段长度多态性方法，检测92例PCAD患者(PCAD组)和95例年龄及性别相匹配的非冠心病者(对照组)的rs2285053(-735C/T)、rs3918242(-1562C/T)基因的单核苷酸基因多态性，判定其基因型并统计各基因型及等位基因的频率。ELISA法检测血浆MMP9的水平。结果 MMP2 rs2285053位点多态性在PCAD组和对照组中的基因型分布和等位基因频率差异无统计学意义(χ2=1.33，P=0.249)。MMP9 rs3918242位点PCAD组C/T+T/T型高于对照组(χ2=6.22，P=0.013)，T基因频率亦高于对照组，有显著性差异(χ2=7.75，P=0.005，OR=2.66)。早发急性冠脉综合征组(premature acute coronary syndrome, PACS)C/T+T/T型高于对照组，与早发稳定性心绞痛相比差异无统计学意义(χ2=9.11，P=0.003;χ2=2.29，P=0.13)，早发稳定性心绞痛与对照组相比差异亦无统计学意义(χ2=1.3，P=0.254)。Logistic回归分析显示，MMP9 rs3918242位点携带T等位基因为PCAD发病的独立危险因素。结论 MMP2 rs2285053(-735)位点多态性可能与PCAD的发病无相关性，MMP9 rs3918242位点可能与PCAD及PACS发病相关，rs3918242(-1562)T等位基因可能是PCAD的遗传易感基因。 【关键词】 早发冠心病;基质金属蛋白酶;单核苷酸基因多态性 　　Medical School of Xian Jiaotong University, Xian 710004, China)ABSTRACT: Objective To investigate the association of matrix metalloproteinase2 gene rs2285053 (-735C/T) and matrix metalloproteinase9 gene rs3918242(-1562C/T) polymorphisms with premature coronary artery disease (PCAD) in Han population of Shaanxi Province. Methods The polymorphisms of gene MMP2-735C/T and MMP9-1562C/T were measured by polymerase chain reaction and restriction fragment length polymorphism analysis (PCRRFLP) in 92 patients with PCAD (PCAD group) and 95 noncoronary heart disease patients (control group), who were matched in age and sex. Plasma level of MMP9 was measured by enzymelinked immunosorbent assay (ELISA). Results The frequencies of MMP2-735C/T gene polymorphism in PCAD group were not significantly different from those in control group (P=0.249). The frequencies of gene polymorphism T allele were significantly higher in PCAD group than those in control group (P=0.005, OR=2.66). The frequencies of the three genotypes (CC, CT a