一株高效降解菲的植物内生细菌筛选及其生长特性.pdfVIP

中国环境科学 2013,33(1) ：95~102 China Environmental Science 一株高效降解菲的植物内生细菌筛选及其生长特性 * 刘 爽,刘 娟,凌婉婷,朱雪竹,高彦征 (南京农业大学,土壤有机污染控制与修复研究所,江苏 南京 210095) 摘要：从长期受PAHs 污染的植物看麦娘中分离出 1 株可高效降解菲的内生细菌 Pn2,经生理生化特征分析和 16S rDNA 序列同源性分析, 初步鉴定为Naxibacter sp.,研究了菌株 Pn2 的生长特性及其对菲的降解作用.结果表明,菌株 Pn2 能以菲为唯一碳源生长,并对菲有良好的降 解性能.菲浓度为 49.92mg/L 时,30℃下 150r/min 振荡培养 72h,菲降解率高达 98.78%.接种量和污染强度显著影响 Pn2 对菲的降解:接种量 越大,菲降解率越高;随污染强度升高,菲降解率先增大后减小,最适污染强度为 150mg/L.菌株 Pn2 有较强的环境适应能力.温度为 25~37℃、 环境 pH 值为 6.0~8.0、盐浓度 1%~2%范围内,菌株 Pn2 生长状况良好.菌株 Pn2 为好氧生长,通气量越大,菌株生长越旺盛.菌株 Pn2 对低浓 度的氨苄青霉素和氯霉素有抗性. 关键词：植物内生细菌；筛选；菲；生长特性；降解 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2013)01-0095-08 Isolation and characteristics of an efficient phenanthrene-degrading endophytic bacterium strain from plants. * LIU Shuang, LIU Juan, LING Wan-ting, ZHU Xue-zhu, GAO Yan-zheng (Institute of Organic Contaminant Control and soil Remediation, Nanjing Agricultural University, Nanjing 210095, China). China Environmental Science, 2013,33(1) ：95~104 Abstract ：An efficient phenanthrene-degrading endophytic bacterial strain Pn2 was isolated from Alopecurus aequalis Sobol grown in PAH-contaminated soils. Pn2 was identified as Naxibacter Sp. based on physiological characteristics and 16S rDNA Sequence homology analysis. The experiments on phenanthrene-degrading features and growth characteristics of the strain showed that strain Pn2 can utilize phenanthrene as the sole carbon source. At 30℃ and 150 r/min, the degrading ratio of phenanthrene (49.92mg/L) in solution was 98.78% after 72 hours rotary culture. The inoculation amount and pollution intensity positively influenced the phenanthrene degradation: the larger amount of Pn