一株高效异养硝化菌的分离、鉴定及其氨氮去除特性.pdfVIP

中国环境科学 2013,33(7) ：1309~1315 China Environmental Science 一株高效异养硝化菌的分离、鉴定及其氨氮去除特性 吴建江,王兆阳,许培雅* (浙江工业大学生物与环境工程学院,浙江 杭州 310014) 摘要：以丁二酸钠为唯一 C 源,从垃圾渗滤液活性污泥中富集、分离及筛选出一株高效的异养硝化菌,通过形态观察、生理生化特征及 16S rDNA 序列分析,对分离菌株进行了鉴定, 同时对其硝化功能和氨氮去除特性进行了研究.结果表明,分离到的异养硝化菌为假单胞菌属 (Pseudomonas sp.),命名为 XS76,其 GenBank 登录号为(JQ934897).该菌经过 24h 培养,有约 60%的氨氮转化为胞内氮,35%左右的氨氮被去除, 仅有少量硝酸盐氮的积累,没有羟胺和亚硝酸盐氮的积累.碳源、有机氮源、C/N 比、温度等因素均对氨氮去除有较大的影响,在接种量为 4% 、丁二酸钠为碳源、硫酸铵为氮源、C/N 15、转速 180~200r/min、pH 6~9 及温度为 34℃,氨氮负荷为 420 mg/L 时处理效果最佳, 96 h 氨氮去除率可达 99.20%.显示了高效的氨氮去除效果,具有潜在的实际废水应用价值. 关键词：异养硝化菌；筛选；鉴定；去除特性 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2013)07-1309-07 Isolation and identification of an efficiently heterotrophic nitrifier, and its removal characterization of ammonia * nitrogen. WU Jian-jiang, WANG Zhao-yang, XU Pei-ya (1.College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310004, China). China Environmental Science, 2013,33(7)：1309~1315 Abstract ：A heterotrophic nitrifying bacteria isolated from the activated sludge of landfill leachate through the enrichment medium with sodium succinate as the sole carbon source was identified by its morphologic observation and the analysis of physiological characteristics and 16S rDNA sequence; its nitrifying function and removal characterization of ammonia nitrogen were also studied in this paper. This strain was identified as Pseudomonas sp., named as XS76 with JQ934897 as its GenBank accession number. After 24h-cultivation, nearly 60% ammonia nitrogen was converted into intracellular nitrogen and 35% removed. There was only a small amount of nitrate nitrogen accumulation, but with no accumulation of hydroxylamine and nitrite nitrogen. The factors including carbon source, o