羟乙基淀粉13004对全脑缺血再灌注大鼠血脑屏障的影响.docVIP

羟乙基淀粉130/0.4对全脑缺血/再灌注大血脑屏障的影响摘要目的：6%中分子羟乙基淀粉130/0.4（HES130/0.4）对全脑缺血/再灌注大鼠血脑屏障（BBB）的，探HES130/0.4影响血脑屏障的可能机制。采用3血管阻断法建立全脑缺血/再灌注模型假手术组（S组）、 缺血/再灌注组（R组）、羟乙基淀粉组（H组）。脑缺血前 10min、脑缺血10min、再灌注后10min采集股动脉血监测动脉血氧分压和二氧化碳分压。阻断前10min,再灌后0min及处死前10min分别检测血细胞比容Hct）。组织含水量伊文思蓝含量核转录因子-κBNF-κB）和肿瘤坏死因子-α（TNF-α）组血气指标比较差异无统计学意义组Hct间无明显差异。与S组相比较，R组脑含水量伊文思蓝含量增加（P0.05），H组差异无统计学意义；与H组比较，R组脑含水量伊文思蓝含量增加（P0.05）与S组相比，R组、H组的NF-κB TNF-α活性升高（P0.01）,与H组相比，R组的NF-κB TNF-α活性升高HES130/0.4能够减轻全脑缺血/再灌注BBB的破坏，降低其通透性，减轻脑水肿，具有，其作用与抑制TNF-α和NF-κB的表达，减轻炎症损伤有关。核转录因子-κB肿瘤坏死因子-αEffect of Hydroxyethyl Starch 130/0.4 on Global Cerebral Ischemia/reperfusion BBB in Rats YE Wei -guang , Wang Tian-long，et al. Department of Anesthesiology, Xuanwu Hospital, capital medical university, Beijing 100053, China 【Abstract】 Objective To observe the effects of Hydroxyethyl Starch 130/0.4 on global cerebral ischemia/ reperfusion in rats and to investigate the effect of HES130/0.4 of the underlying mechanism. Methods Forty eight SD rats were divided randomly into 3 groups of sham operation (S group), Ischemia Reperfusion group (R group), HES130/0.4+ I/R group (H group). The blood gas and blood hematocrit analysis were performed during the experiment. The content of TNF-α、EB、 Brain water content and NF-κB activity of brain tissue was determined at 12hours after the experiment. Result The change of blood gas and blood hematocrit is normal at each group. The content of TNF-α、EB、 Brain water content and NF-κB activity were improved in R group and H group compared with those in S group. The content of TNF-α、EB、 Brain water content and NF-κB activity were improved in R group compared with those in H group. Conclusion HES130/0.4 can lighten ischemia-reperfusion BBB injury in ischemia-reperfusion rat. HES130/0.4 have cerebral protection, the underlying mechanism may concerned with decreased TNF-α and NF-κB expression, attenuate inflammation injury. 【Key words】 Reperfusion injury; Blood brain barrier; Nuclear factor-kappaB; Tumor