肝细胞癌中CHFR、EDNRB和3OST2基因异常甲基化的研究.DOCVIP

临床医学论文-肝细胞癌中CHFR、EDNRB和3OST2基因异常甲基化的研究 ???????????? 作者：谢坤 朱立新 耿小平 李晓明 熊奇如 孙昀 刘念　　? 【摘要】? 目的 研究肝细胞癌中细胞有丝分裂前期检查点(checkpoint with forkheadassociated and ring finge,CHFR)基因、内皮素B受体(endothelin receptor B,EDNRB)基因和3O硫基转移酶2(heparan sulfate Dglucososaminyl 3Osulfotransferase2,3OST2)基因启动子区异常甲基化状态及其临床意义。方法 收集癌组织及相应癌旁组织标本40例，以及14例肝血管瘤患者肝脏和2例肝移植供肝标本，并排除乙肝病毒感染和肝硬化。使用甲基化特异性PCR反应检测上述标本中CHFR、EDNRB和3OST2基因的甲基化状态。结果 CHFR基因在癌组织和癌旁组织的甲基化率分别为52.5%和27.5%，癌组织中的甲基化率高于癌旁组织(P0.05)。EDNRB基因在癌组织和癌旁组织中的甲基化率都为27.5%。3OST2基因在癌组织中甲基化率为12.5%，癌旁组织没有发现甲基化。16例正常肝组织中没有发现上述3种基因的异常甲基化。癌组织中CHFR基因甲基化与非甲基化患者相比，男性多于女性。癌旁组织中EDNRB基因甲基化患者肿瘤的包膜出现率低于非甲基化患者。癌组织中3OST2基因甲基化患者的年龄和肿瘤直径大于非甲基化患者。结论 肝细胞癌中CHFR基因、EDNRB基因启动子的高甲基化是普遍现象，而3OST2基因启动子甲基化率较低。 【关键词】? 肝肿瘤； 基因； DNA甲基化 ??? 【Abstract】? Objective? To study status of the promoter hypermethylation of checkpoint with forkheadassociated and ring finger (CHFR), endothelin receptor B (EDNRB) and heparan sulfate Dglucosaminyl 3Osulfotransferase2 (3OST2) genes in hepatocellular carcinoma and observe its clinical significance. Methods? Methylationspecific PCR was adopted to investigate promoter methylation of CHFR, EDNRB and 3OST2 genes in specimens from 40 cases with hepatic cell carcinoma (HCC), liver tissues from 14 with liver hemangioma and 2 donor livers. Results? The fmethylation rates of CHFR, EDNRB and 3OST2 was 52.5%, 27.5% and 12.5% respectively in HCC tissues and 27.5%, 27.5% and 0% respectively in nontumor tissues. Normal liver had no methylation of 3 genes. Methylation of CHFR was more frequent in HCC tissues than that in nontumor liver tissues. Methylation of CHFR in males was more than that in females. The cases with methylation of 3OST2 gene were older and had larger diameter of carcinoma than those without.Conclusion? Promoter methylation of CHFR and EDNRB genes is a common event in HCC, while frequency of promoter methylation of 3OST2 is rather low. ??? 【Key words】? Hepatic cell carcinoma;?? Genes;?? DNA methylation ??? 我国