米索前列醇对去势后大鼠早期骨丢失的保护.DOCVIP

临床医学论文-米索前列醇对去势后大鼠早期骨丢失的保护 　【摘要】 目的 通过对去势后大鼠给予米索前列醇，了解米索前列醇对去势后大鼠早期骨丢失的保护。方法 选取鼠龄90d的Wistar雌性大鼠共30只，分为正常对照组（对照组）、去势组（去势组）、去势给米索前列醇组（去势给药组）。去势后第2d，去势给药组予以2mg/（kg?d）剂量的米索前列醇生理盐水溶液灌胃，其余两组给予相同体积的生理盐水灌胃，于术后28d对各组的骨密度进行检测，并取第1腰椎脱钙切片，对各组骨小梁进行形态学分析。结果 骨密度检测结果显示:对照组最高，去势给药组次之，而去势组最低，3组之间两两比较差异均有显著性（P0.05）;骨小梁面积:对照组最高，去势给药组次之，去势组最低，3组之间两两比较差异均有显著性（P0.05）;骨保护素的表达量:对照组最高，去势给药组次之，去势组最低，3组之间两两比较差异均有显著性（P0.05）。结论 米索前列醇可抑制去势后雌性大鼠骨量的丢失，其机制可能是通过刺激骨保护素的表达及分泌，来减少骨量的丢失。 　　关键词 米索前列醇 骨密度 骨保护素 逆转录聚合酶链反应????? 　　The effect of misoprostol on the bone of ovariectomized rats?????? 　　【Abstract】 Objective To investigate the influence of misoprostol on bone mineral density（BMD）in ovariecˉtomized（OVX）rats.Methods 30female Wistar rats aged90d were divided into3groups:Sham，OVX，OVX+misoprostol with10rats in each group.The OVX+misoprostol group was treated orally2mg/（kg?d）misoprostol after28days.All rats were sacrificed for treatment，and BMD was measured by dual-enery X-ray absorption，and reˉverse transcription-polymerase chain reaction（RT-PCR）examination indicated the expression of OPG on mRNA level in tibial metaphyses.The first lumbar vertebrae was processed for histomorphometric analysis.Results The BMD of rats show that Sham group was the highest one，while the OVX+misoprostol group was higher than OVX group.There was significant different between the Sham group，OVX group and OVX+misoprostol group.The OPG of3groups of rats showed significantly differenttoo.OVX+misoprostol group was higher than OVX group，and OVX group higher than Sham group.Parameters of bone histomorphometric analysis showed that Sham group was the highest one，while the OVX+misoprostol group was higher than OVX group.Conclusion Misoprostol can effectively preˉvent osteoporosis in ovariectomized rats by up-regulation OPG mRNA level and inhibit osteoclastic bone resorption. 　　Key words misoprostol bone mineral density osteoprotegerin revevse transcriptiou-polymerase chain reˉaction?????? 　　骨质疏松症（Osteoporosis）是以骨量减少，致使骨的脆性增加而易发