晚期糖基化终产物对高糖诱导NRK52E细胞的转分化作用.DOCVIP

临床医学论文-晚期糖基化终产物对高糖诱导NRK52E细胞的转分化作用 【摘要】? 目的： 探讨晚期糖基化终产物（AGEs）对高糖诱导大鼠肾小管导管上皮细胞株NRK52E的转分化干预作用。方法： 大鼠肾小管导管上皮细胞株设空白组（不给葡萄糖）、高糖组（25 mmol/L葡萄糖）、AGEs干预组（100 mg/L AGEs）、高糖AGEs干预组（100 mg/L AGEs+25 mmol/L葡萄糖），培养72 h后，用Western bolt检测转化因子β1（TGFβ1）、Ⅰ型胶原、金属蛋白酶2（MMP2）、α平滑肌肌动蛋白（αSMA）及甲状旁腺素相关肽（PTHrP）在NRK52E细胞的表达，活性氧（ROS）含量应用CM2H2DCFDA试剂盒检测。结果： 高浓度葡萄糖上调TGFβ1、Ⅰ型胶原、MMP2、αSMA以及PTHrP表达，AGEs显著上调高浓度葡萄糖状态下TGFβ1、Ⅰ型胶原、MMP2、αSMA和PTHrP表达，提高ROS含量。结论： AGEs可以促进高浓度葡萄糖诱导肾小管导管上皮细胞转分化。 【关键词】? 糖基化终产物，高级； NRK52E 细胞； 葡萄糖； 受体-转化生长因子β； 胶原I型； 基质金属蛋白酶； 肌动蛋白类； 甲状旁腺激素肽（1-34） 　　［Abstract］Objective: To study the intervention effect of advanced glycation endproducts (AGEs) on hyperglycemia induced transdifferentiation in NRK52E cells. Methods: Four groups of NRK52E cells were established: blank control group (group CON, was given no glucose and no intervention), high glucose group (group HG, given 25mmol/L of glucose), AGEs treated group (group AGE, given 100 mg/L of AGEs), and AGEs plus high glucose treated group (group HGAGE, given 100 mg/L of AGEs plus 25 mmol/L of glucose), and Western blot was carried out to determine the expression of transforming growth factor β1 (TGFβ1),? typeⅠcollagen, metalloprotease 2 (MMP2), smooth muscle actin α (αSMA), and parathyroid hormonerelated peptide (PTHrP) in these cells after 72 h of reaction.Active oxygen(ROS) contents were detected by using CM2H2DCFDA kit. Results: The expression of TGFβ1, typeⅠcollagen, MMP2, αSMA and PTHrP were upregulated by glucose of high concentration; AGEs upregulated TGFβ1, typeⅠcollagen, MMP2, αSMA, and PTHrP under high glucose condition, and increased ROS content. Conclusion: AGEs could increase transdifferentiation induced by hyperglycemia in NRK52E cells. 　　［Key words］ glycosylation end products, advanced; NRK52E; glucose; transdifferentiation; receptors, transforming growth factor beta; collagen type I; matrix matalloproteinases; actins; teriparatide??? 　　晚期糖基化终产物（AGEs）在糖尿病并发症的发生、发展中起着重要的作用。肾小管上皮细胞转分化是肾脏纤维化的重要病理基