瘦素对肝细胞三酰甘油沉积的影响.DOCVIP

临床医学论文-瘦素对肝细胞三酰甘油沉积的影响 【摘要】? 目的 观察瘦素对人L02脂肪变肝细胞三酰甘油(TG)沉积的影响，探讨瘦素在非乙醇性脂肪性肝病发生、发展中的作用。方法 人肝L02细胞培养接种于50 ml 培养瓶中，用50%胎牛血清1640培养基造模，时间24 h。设立正常组、模型组、瘦素处理1，2，3组，处理组瘦素浓度分别为10-7 mol/L，10-6 mol/L，10-5mol/L，分别处理6 h；再以10-6 mol/L 浓度瘦素建立时效关系分别处理（6，12及24 h）。每组均行油红“O”染色观察脂肪化情况，高效液相色谱测细胞内TG含量变化。结果 油红“O”染色显示50%的胎牛血清的1640培养基24 h 能成功地制造肝细胞脂肪变性模型，加入瘦素处理后脂肪化程度减轻。高效液相色谱显示细胞内TG明显减少，且与瘦素呈剂量、时间依赖性关系。结论 肝细胞脂肪变性时，瘦素能使肝细胞脂肪化程度减轻，能减少肝细胞TG的沉积,对脂肪肝防治有一定的作用。 【关键词】? 脂肪变肝细胞；瘦素；三酰甘油 　　Effects of Leptin on Disposition of Triglyceride in Fatty Liver Cells 　　Zhou Hongyu,Yang Xuefeng? 　　(Department of Gastroenterology, Second Affiliated Hospital, Nanhua University, Hengyang 421001, China) 　　Abstract： Objective? To explore the effect of leptin on triglycerides disposition in normal and nonalcoholic induced fatty liver cell. Methods? L02 liver cells were cultured in 50 ml culture dish. Fatty liver cell models were made with 50% inactivated embryo blood serum for 24 hours.Results? Oil red staining showed that 50% inactivated embryo blood serum can make fatty liver cell model. After L02 liver cells were turned into fatty liver cells, leptin was added to fatty liver cells. The HPLC showed that the model groups triglyceride disposition increased clearly, but after leptin was given into the model cells, triglycerides disposition decreased obviously, which was? positive correlated with leptins concentration. Conclusion? When liver L02 cells were turned into? fatty liver cell, leptin can inhibit triglycerides disposition. Leptin has some therapeutic effect on fatty liver cells. 　　Key? words： fatty liver cell; leptin; triglyceride ??? 非乙醇性脂肪肝（NAFLD）是一种肝组织学改变与乙醇性肝病相类似，但与饮酒无明确关系的临床病理综合征，根据其病理学改变及临床表现分为单纯性脂肪肝、脂肪性肝炎、脂肪性肝纤维化、脂肪性肝硬化[1,2]。肝脏是体内参与脂质代谢的重要场所，肝脏选择性地摄取血中的非饱和脂肪酸，经酯化合成三酰甘油（TG），TG在肝细胞内，一方面氧化供能，另一方面分泌入血转化为低密度脂蛋白（LDL）和高密度脂蛋白（HDL）被其他组织利用。肝脏所含脂质绝大部分为TG，各种致病因素可导致肝细胞内TG异常堆积而导致脂肪肝（FLD）的发生。瘦素是肥胖基因（obese gene）编码的蛋白产物，其受体广泛分布于脂肪、骨骼肌、肝脏、心、肺、胰、卵巢、睾丸等外周组织。瘦素通过与受体结合而发挥作用[3]。瘦素的主要作用是调节机体脂肪的稳定，抑制食欲、增加能耗和减少体质量。本研究以人肝L