C-MYC、突变型P53蛋白在小细胞肺癌中的表达及意义.DOCVIP

临床医学论文-C-MYC、突变型P53蛋白在小细胞肺癌中的表达及意义 【关键词】? C-MYC蛋白；P53蛋白；小细胞肺癌；免疫组织化学 ????? ［摘要］? 目的? 研究C-MYC、突变型P53蛋白在小细胞肺癌（SCLC）中的表达并分析其与SCLC临床病理学特征及预后之间的关系。方法? 用免疫组化的方法检测30例原发灶及10例正常肺组织中C-MYC、突变型P53蛋白的表达水平，按阳性表达率进行χ2分析。结果? SCLC中C-MYC、突变型P53蛋白表达显著高于正常肺组织。两者阳性表达都与患者的生存期及SCLC分化程度呈负相关（P0.05），与SCLC的淋巴结转移呈正相关（P0.05），与患者的年龄、肿瘤大小无关（P0.05）。C-MYC 蛋白表达与肿瘤p-TNM分期呈正相关（P0.05），而突变型P53蛋白表达与肿瘤p-TNM分期无相关性（P0.05）。结论? SCLC存在C-MYC、突变型P53蛋白过度表达，提示它们在SCLC发生、发展过程中可能起着重要作用。 ??? ［关键词］? C-MYC蛋白；P53蛋白；小细胞肺癌；免疫组织化学 ??? ［Abstract］? Objective? This study observed the expression of C-MYC and mutated P53 protein in SCLC,in order to analyse the relationship between their expression and prognosis of the patients as well as clinicpathological characteristic.Methods? The immunohistochemical staining method,strepatavidin-peroxidase（S-P）method was used to detect the C-MYC and mutated P53 protein in 30 cases of SCLC.10 normal lung tissues were contrasted.The positive expression was analyzed with χ2 test.Results? The expression level of the C-MYC and mutated P53 in cancer tissues were significantly higher than that in the normal lung tissues.Their expression were negatively correlated with survival time and differentiation degree （P0.05）,positive correlated with lymph nodes metastasis.They were not correlated with age and the size of tumor （P0.05）.There was a positive correlation between C-MYC protein and p-TNM stages of the cancer （P0.05）,but mutated P53 protein was not correlated with p-TNM （P0.05）.Conclusion? There are the overexpression of C-MYC and mutated P53 protein in SCLC,which suggests that they may play an important role in the occurrence development of SCLC. ??? ［Key words］???? C-MYC protein;P53 protein;SCLC;immunohistochemistry ??? 目前人们对非小细胞中的P53基因研究较多，而对小细胞肺癌中P53基因、C-MYC基因的表达及两者之间关系的相关性研究，国内外文献仍较少。本研究应用免疫组化技术对30例小细胞肺癌组织突变型P53、C-MYC蛋白的表达水平进行分析，以探讨突变型P53及C-MYC蛋白表达水平与小细胞肺癌发生发展的关系，并用SPSS软件包进行检验两种蛋白的表达水平与小细胞肺癌临床病理学特征及预后之间的关系。 ??? 1? 资料与方法 ??? 1.1? 一般资料? 收集我院1989～2005年手术切除的30例小细胞肺癌标本。男27例，女3例；年龄25～70岁，平均51.2岁；肿瘤