正交试验优选复方通脉胶囊的提取工艺.docVIP

正交试验优选复方通脉胶囊的提取工艺 张帅1*，郭魏魏2#，申雷3（1.郑州市儿童医院药学部，郑州 450000；2.郑州大学第五附属医院药学部，郑州 450000；3.解放军第153医院药剂科，郑州 450000） 摘 要：目的：优选复方通脉胶囊的最佳提取工艺。方法：高效液相法测定丹参酮IIA的含量, 采用正交设计法考察煎煮次数、煎煮时间、加水量三因素对水提工艺提取成分提取率的影响;考察醇沉浓度、醇沉前水提液相对密度、醇沉时间三因素对醇沉工艺有效成分含量的影响。结果：煎煮次数、加水量对水提工艺有显著影响(P0.05); 醇沉浓度、醇沉前水提液相对密度对醇沉工艺有显著影响(P0.05)。结论：最佳水提醇沉工艺为药材加10倍量水，煎煮2次，每次1.5h；水提液浓缩至相对密度为1.10，加入乙醇使含醇量至70%，静置24h。 关键词：复方通脉胶囊；正交试验；提取工艺；丹参酮IIA Optimization of the Extraction Technology of Co Tongmai Capsule ZHANG Shuai 1*，GUO Wei-wei2#，SHEN Lei3（1. Childrens Hospital Department of pharmacy of Zhengzhou, Zhengzhou 45000;2. The Fifth Affiliated Hospital of Zhengzhou University of pharmacy,Zhengzhou 45000;3. The 153rd Central Hospital of PLA pharmacy, Zhengzhou 45000） ABSTRACT OBJECTIVE：To optimize the extraction technology of Co Tongmai Capsule. METHODS：The content of tectoridin was detected by HPLC. Effects of three factors, extraction time、extraction times、water volume on extraction rate were evaluated by orthogonal design. Effects of three factors, alcohol precipitation concentration、concentration of condensed solution、precipitation time on content of tectoridin of alcohol precipitation were also evaluated by orthogonal design. RESULTS：Extraction times and water volume had a significant effect on water-extraction(P0.05); concentration of condensed solution had a significant effect on alcohol-precipitation (P0.05). CONCLUSION: The optimized extraction technology was as follows: 10 volumes of water were added to 1 volume of the crude drug, followed by decoction for 2 times, each decoction lasting 1.5h. The solution of water-extraction was concentrated to a relative density of 1.10 and then precipitated by alcohol at the concentration of 70%,then keeping still for 24h. KEY WORDS: Co Tongmai Capsule; Orthogonal design; Tectoridin; Water-extraction and alcohol-precipitation; Tanshenone IIA 丹参为唇形科植物Salvia miltiorrhizaBge 干燥根及根茎, 临床用于治疗冠心病、心绞痛、缺血性中风、栓塞、肝脾肿大、化脓性感染等疾病。现代药理学研究表明, 丹参的有效成分分为脂溶性的菲醌类衍生物和水溶性的酚酸类成分, 前者为抗菌抗炎的主要成分, 其