肉苁蓉属4种植物的高效液相色谱指纹图谱研究.pdfVIP

4 4 肉苁蓉属44种植物的高效液相色谱指纹图谱研究 1,2 1 * 1 2 3 刘友刚 ，徐 荣 ，陈 君 ，王 威 ，刘同宁 1(中国医学科学院 北京协和医科医学院 药用植物研究所，北京100193) 2(天津中医药大学，天津 300193) 3（宁夏永宁县本草苁蓉种植基地，银川 750100） 摘要：目的：建立肉苁蓉属不同植物的高效液相（HPLC）指纹图谱。方法：采用phenomenex (250×4.6mmI.D.489519-6，5μm)色谱柱，流动相乙腈(A)和0.5%醋酸水(B)，采用多台阶式梯度 洗脱法，0～18min (4%A—12%A)，18～40min(12%B—15%B)，40～55min (15%—20%B)， 55～65min (20%A)，65～68min(20%A—4%A)，流速0.9mL/min，检测波长310nm，柱温35℃， 进样量为10μL。结果：四种植物共有7个共有指纹峰，可以指认两个共有峰。结论：从整体上 显示肉苁蓉属四种植物的特征，为肉苁蓉药材的质量控制提供有效手段。 关键词：肉苁蓉属；高效液相色谱；指纹图谱 StudiesonHPLCfingerprintof4speciesofgenusCistanche StudiesonHPLCfingerprintof4speciesofgenusCistanche SSttuuddiieessoonnHHPPLLCCffiinnggeerrpprriinnttooff44ssppeecciieessooffggeennuussCCiissttaanncchhee 1,2 1 1* 2 3 LiuYou-gang ,XuRong ,ChenJun ,WangWei ,LiuTongning 1 (TheInstituteofMedicinalPlantDevelopment, ChineseAcademyofMedicalSciencesandPekingUnion MedicalCollege, Beijing, 100193) 2 (Tianjin University ofTraditionalChinese Medicine 3001933. ) 3 NingxiaYongningPlantationofHerbaCistanche,Yinchuan750100,China) [ABSTEACT] OBJECTIVE: [ABSTEACT] OBJECTIVE: [[AABBSSTTEEAACCTT]] OOBBJJEECCTTIIVVEE:: To establish the HPLC fingerprint of Cistanche deserticola. METHODS: METHODS: MMEETTHHOODDSS:: RP-HPLC was used on phenomenex C column(250 mm x 4.6 mm, 5μm)with the 18 gradientelution solventsystem composed of acetonitrile-0.5％ Acetic acid water solution, thecolumn temperature was 35 °C, the detection wavelength was 310 nm, and sample injection was 10μL. RESLTS： RESLTS： RREESSLLTTSS：：HPLC fingerprintofCistanchedeserticola.wasestablished. 7 common peaks wereselected CONCLUSION: CONCLUSION: as the fingerprint peaks of Cistanche deserticola. CCOONNCCLLUUSSIIOONN:: This method is simple, credible andofgoodreproducibilit