茶叶内生菌与分离鉴定及其生防功能初探.docVIP

茶叶内生菌的分离鉴定及其生防功能初探 朱育菁，陈 璐，蓝江林，苏明星，刘 波( 福建省农科院农业生物资源研究所，福建 福州 350003 摘要：对采自福建宁德地区的大白毫和福云六号茶叶进行内生菌的分离和脂肪酸鉴定。从7份样品中共分离到16株细菌1株真菌。老叶中均未检测到内生菌，芽叶中内生菌数量为26.×106 -139.5×106 cfu/g。有机种植的福云六号芽叶的含菌量是大白毫的1.94倍。常规种植的大白毫芽叶的含菌量为97.5×106139.5×106 cfu/g，明显高于有机种植的26.5×10628.3×106 cfu/g。大白毫带有红杆菌属、微杆菌属、根瘤菌属和贪噬菌属的内生细菌，福云六号仅含有根瘤菌属和贪噬菌属。有11株茶叶内生菌对供试10种病原菌表现出拮抗活性，其中放射根瘤菌Eb659菌株抑菌谱最广，具有开发作为生防菌防治植物病害的潜质。 关键词：茶叶；内生菌；分离；脂肪酸鉴定；生物防治 中图分类号：Q93-331; Q939.95 文献标识码：A 文章编号： Isolation, identification and the biocontrol potential of endophyte in theasienensis (Camellia sinensis) ZHU Yu-jing, CHEN Lu, LAN Jiang-lin, SU Ming-xing, LIU Bo (Agricultural Bioresource Institute, Fujian Academy of Agricultural Sciences，Fuzhou, Fujian 350003，China) Abstract：The isolation, fatty-acid identification and inhibition ability against pathogeny of the endophyte in theasienensis (Camellia sinensis) were conducted. The theasienensis samples were collected from Linde area, Fujian Province; the varieties of the theasienensis were Dabaihao and Fuyunliuhao. 16 strains of endophytic bacteria and 1 endophytic fungus were isolated from 7 theasienensis samples. None of endophyte was detected in the old leaves of both theasienensis, whereas the endophytic bacteria in the buds were 26.5×106 -139.5×106 cfu/g. The endophytes in bud of Fuyunliuhao was 1.94 times more than those of Dabaihao, while both theasienensis were treated with organic planting. However, the endophytes in “Dabaihao” buds treated with normal planting were 97.5×106 -139.5×106 cfu/g, obviously higher than 26.5×106 -28.3×106 cfu/g of the buds treated with organic planting. Dabaihao contained endophytic bacteria belonging to Rhodobacter, Microbacterium, Rhizobium and Variovorax, whereas the Fuyunliuhao had only Rhizobium and Variovorax. 11 strains of the endophytic bacteria in theasienensis showed inhibition abilities to 10 pathogenies tested. Eb659 (R. radiobacter) had the biggest inhibition spectrum, which showed that it might have potential to be