同种异体骨异位再血管化与实验研究.docVIP

同种异体骨异位再血管化的实验研究 周明武1，3，罗彦平2，杨瑞甫3，宋 力3，李 扬2，张 迅2，徐立博2，王义生1 （450003 郑州，郑州大学第一附属医院骨科1；453003 河南 新乡，新乡医学院 在读研究生 2；450007 郑州，解放军第一五三中心医院全军创伤骨科中心3） [摘要] 目的 比较不同时间段兔同种异体胫骨与自体大段胫骨异位再血管化程度的差异性。方法 健康成年（6月龄）中国白兔70只，体质量（2.5±0.5）kg，按随机数字表法分为实验组（同种异体骨组）30只、对照组(自体骨组)30只，余10只用作制备同种异体骨供体。实验组将制备好的1.5 cm长兔异体胫骨段置于兔大腿隐动脉处的股直肌与股内侧肌的间隙内，1.0克氏针固定于股骨上。对照组取与实验组相同部位等长自体胫骨同法置于兔大腿相应部位。分别于术后4、8、12周通过大体标本观察、免疫组化法检测移植骨组织血管内皮生长因子 (vascular endothelial growth factor, [通信作者] 王义生，E-mail:zmw153@126.com Experimental study of ectopic revascularization about allograft segment Zhou Mingwu1，3 ,Luo Yanping2 ,Yang Ruifu3, Song Li3, Li Yang2, Zhang Xun2 , Xu Libo2 ,Wang Yisheng1 （The First Affiliated Hospital of Zhengzhou University, Zhengzhou of Henan,450003, China; Xinxiang Medical University, Xinxiang of Henan,453003;Orthopedic Trauma Center of PLA，the 153th Hospital of PLA，Zhengzhou of Henan,450007,China） [Abstract] Objective At different time periods，Comparison of the difference about ectopic revascularization between autogenous bone graft and allograft from large segment tibia of rabbits. Methods With methods of random grouping, the 60 Healthy adult Chinese rabbits ( 6 months of age ), weighing 2.5±0.5kg, were divided into experimental group ( allogeneic bone group ) and the control group ( autograft group ), 30 rabbits in each group. Another 10 rabbits were allogeneic bone donor. In experimental group, when 1.5cm long rabbit tibial allograft were finishied, they were implanted into spatium intermusculare between the musculus rectus femoris and medial vastus muscle of the rabbit around the saphenous artery and were fastened to the femur by 1.0 Kirschner-wire. In control group, autologous tibias were done, the same as experimental group including length and position and method. 4weeks and 8 weeks and 12 weeks Postoperative, respectively, the postmortem specimens were examined gross and immunohistochemistry and the expression of VEGF and CD34 of transplanted bone tissue were detected. Resu