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天然产物研究与开发 N at Prod Res Dev 2006 ,18 :787791 ( ) 文章编号 2006 　 　 由环己酮生物合成己内酯的研究 郑爱芳1 ,2 ,李大平1 ( 1. 中国科学院成都生物研究所 ,成都 610041 ;2 . 中国科学院研究生院 ,北京 100039) 摘 　要 :从本实验室保藏菌种中分离筛选出一株能够以环己酮作为唯一碳源的菌株 ,初步鉴定为邻单胞菌属 , 并对其产物进行 GC/ MS 定性 ,发现有己内酯生成 ,存在 Baeyervilliger 反应 。文章还探讨了p H ,装液量 ,底物浓 度 ,培养时间 ,温度以及转速等条件对细菌生长的影响 ,并进一步研究了细菌的底物广谱性 ,发现此菌能够利用 与环己酮具有相似结构的环戊酮等有机物 。 关键词 :环己酮 ; 己内酯 ;邻单胞菌属 ;生物合成 ;Baeyervilliger 反应 中图分类号 :Q933 ;TQ031. 2 文献标识码 :A Study on Biosynthesis of Caprolactone from Cyclohexanone ZHEN G Aifang1 ,2 ,L I Daping1 ( 1. Chengdu Institute of B iology , Chinese Academy of Sciences , Chengdu 610041 ,China ; ) 2. Graduate School of the Chinese Academy of Sciences ,Beijing 100039 ,China Abstract :A strain which can utilize cyclohexanone as sole carbon source was isolated and identified as Plesiomonas sp . ,and in its productions ,caprolactone was found with GC/ MS and Baeyervillager reaction also existed. Effects of p H ,volume ,concentra tion of cyclohexanone ,cultivating time ,temperature and rotate speed on the growth of bacteria were discussed ,and the other or ganic substrates were also studied. The results indicate that the strain can utilize these organic substrates having the similar structure with cyclohexanone such as cyclopentanone etc . Key words :cyclohexanone ;caprolactone ; Plesiomonas sp . ;biosynthesis ;Baeyervillager reaction 　　己内酯单体是一个很有用的化学中间体 ,在合 化在经济