海洋放线菌作为微生物源杀虫剂的试验【毕业论文】.docVIP

本科毕业论文 （20 届） 海洋放线菌作为微生物源杀虫剂的试验 专业：生物科学 目录 摘要 I Abstract II 引言 1 1 材料与方法 2 1.1 样品来源与供试虫源 2 1.2 培养基 2 1.3 海葵共附生放线菌的分离 2 1.4 菌株发酵与发酵液提取、热处理 3 1.5 卤虫毒性实验 3 2 结果 4 2.1 分离到的海葵共附生放线菌 4 2.2 发酵提取液 6 2.3 菌株杀虫活性的筛选结果 6 2.4 菌株DH102发酵提取液杀虫活性的稳定性试验结果 8 3 讨论 9 3.1 海葵共附生放线菌的分离 9 3.2 发酵提取液杀虫活性及其稳定性 9 3.3 海洋无脊椎动物共附生放线菌作为生物农药的潜在价值 10 3.4 海洋杀虫活性物质生物筛选技术的发展方向讨论 10 参考文献 11 致谢 12 摘要 [摘要] 近年来，随着人们对环境污染的重视程度的提高，社会呼吁取代或减少有机杀虫剂使用的要求随之不断高涨。天然产物源于自然，经过无数年的优化组合，可能更适应农业可持续发展的要求，人们转而从自然界寻找新的生物源杀虫剂。从海洋生物中寻找杀虫活性成分是目前杀虫剂研究的热点之一。本文从舟山朱家尖海域的海葵经过分离纯化得到4株共附生海洋放线菌，利用用卤虫毒性测定法进行了杀虫活性菌株的初步筛选以及稳定性的复筛，在初筛后，发现24h后卤虫死亡率高于90%的菌株有DH101，DH102，DH1043株，对杀虫活性最强的1株菌株DH102进行温度稳定性检测，发现菌株发酵提取液对于温度较敏感，在60℃丧失一半的活性而在90℃，杀虫活性消失。实验结果表明表明菌株DH102的发酵液提取产物具有较高的杀虫活性，有作为生物农药资源微生物的研究开发潜力。 [关键词] 生物源杀虫剂；海洋放线菌；杀虫活性； 筛选 Abstract [Abstract] In recent years, as people on the importance of environmental pollution improvement in the level of social calls to replace or reduce the requirement to use organic pesticides followed rising. Natural products derived from nature, after countless years of excellent Of the combination, may be more to adapt to the requirements of sustainable development of agriculture, people turned to nature to find new biological insecticide. From marine organisms in the search for insecticidal active ingredient is one of the hot pesticides. This article from the Zhoushan sea area in the quagmire of Dinghai mud samples obtained after purified 30 marine actinomycetes, with Artemia liquid insecticidal activity determination were the primary screening and secondary screening strains in screening, we found that 24h after the halogen insect mortality rate higher than 90% of the strains had DH102, DH103, DH107, DH109, ??DH201,, DH203, DH204, DH207,, DH210, DH301, DH303, DH306, DH308 and other 13 strains, for which 13 isolates were re-screened, found that several strains of Artemia in the fermentation broth for the ID50 high, but then processed through freeze-thaw, some strains of the insecticidal activity of a sudd