肿瘤型丙酮酸激酶在结直肠癌血浆中的测定及其临床意义.pdfVIP

现代肿瘤医学 2010年 03月 第 18卷第 03期 · 5l1 · 肿瘤型丙酮酸激酶在结直肠癌血浆中的测定及其临床意义 叶维洁 ，金冶宁 StudyonplasmatumortypeM2pyruvatekinaseinpatientswithcoloretalcancerandtheir clinicalvalue YEWei— ie．JIN Ye—ning DepartmentofOncology，ShanghaiRuijinHospital，Shanghai200030，China． A【bstract】 0bjective：Toassessplasmalevel,~oftumorM2pyruvatekinase(TUM2一PK)incolorectalcancer (CRC)patients．Methods：PlasmalevelsofTUM2一PKweremeasuredin16healthydonorsand40patientswith colorectalcancerbyenzyme—linkedimmunosorbentassay(ELISA)(postoperativeplasmahavebeenmeasuredin22 patientsandpostchemotherapyplasmahavebeenmeasuredin7patientswhoacceptedthechemotherapyofXELOX once)．Menawhile，theconcentrationofserumcmeinoembryonicantigen(CEA)hasalsobeendetermined．Results： PlasmaTU M2一PKlevelsinpatientswithCRC、weresignificnatlyraisedcomparedwjt}1thoseinhealhtydonorsfP 0．01)．PlasmaTUM2一PKlevelswereassociatedsingificantlywithDuke~stage(P0．01)，recurrenceanddistnat metastasis(P0．01)．InCRCpatients，preoperativeTUM2一PKlevelswerehigherinpatientswithlymphnodeme— tastasis(P=0．030)，advancedDuke~stage(P=0．048)，poordifferentiation(P=0．407)anddistantmetastasis (P=0．022)．TuM2一PKlevelsidentifiedpatientswithcoloreetalcancerwithasensitivityof80％ at100％ speci— ficity(P0．O1)，patientswithearlycolorectalcancer(DukesA+B)wihtasensitivityof55．6％at100％ specific— ity(P0．01)，whileat100％ specificityserumCEAdid tllasensitivityof52．5％ forallpatientsandwithasen- sitivityof11．1％ f0rDukesA +B patients．CombingCEAandTU M2一PK measurementsmayincreasehtesensitivi— tiesobtainedfrom TU M2一PK alone．TheconcentrationofpostoperativenadpostchemohterapyplasmaTU M2一PK weredecreasedsignificantly(bothP0．01)．Conclusion：P1asmaconcentrationofTUM2一PKwillbeanovel mrakerincolorectal cancer． K【eywords】colorectalcancer；tumorM2pyruvatekinase(TuM2一PK)；cracinoem