蒙药二味杜仲胶囊对去卵巢大鼠子宫雌激素受体的影响.pdfVIP

·334 · Journal Of InnerMongoliaMedical University摇 Aug.2014摇 Vol.36摇 No.4 ·蒙医药论坛 · 蒙药二味杜仲胶囊对去卵巢大鼠子宫雌激素受体的影响 1 2 1 刘摇 岩 ,摇 王久和 ,摇 常摇 虹 (1. 内蒙古医科大学附属医院 中医科,内蒙古 呼和浩特010050;摇 2. 内蒙古自治区中蒙医院) 摇 摇 摘摇 要:目的:研究蒙药二味杜仲胶囊对去卵巢大鼠子宫雌激素受体的影响,探讨蒙药二味杜仲胶囊防治绝 经后骨质疏松症的作用机制。 方法:取4 月龄Wistar雌性大鼠84 只,随机分为假手术组、模型组、己烯雌酚组、 强骨胶囊组、二味杜仲胶囊高、中、低剂量组7 组,每组12 只,确定造模成功正常条件下饲养90d后假手术组和 模型组采用生理盐水、治疗组采用相应药物灌胃,灌胃90d后心内取血,采用免疫组化法观察子宫雌激素受体亚 型ER琢、ER茁的表达。 结果:蒙药二味杜仲胶囊提高了去卵巢大鼠血清雌二醇水平;使大鼠子宫雌激素受体 ER琢、ER茁的表达增强。 结论:蒙药二味杜仲胶囊可提高去卵巢大鼠E2 水平,使雌激素受体ER琢表达增强、进 一步证明蒙药二味杜仲胶囊具有类雌激素样作用;蒙药二味杜仲胶囊使去卵巢大鼠子宫ER茁表达增强,对子宫 内膜无雌激素样刺激增生作用;对去卵巢大鼠骨质疏松有明显的治疗作用。 关键词:绝经后骨质疏松;蒙药二味杜仲胶囊;子宫雌激素受体;去卵巢大鼠 中图分类号:R291.2摇 摇 摇 摇 摇 文献标识码:A摇 摇 摇 摇 摇 文章编号:2095-512X(2014)04-0334-05 EFFECTS OF ERWEIDUZHONG CAPSULE ON OVARIECTOMIZED EXPERIMENTAL RAT UTERINE ESTROGEN RECEPTOR LIU Yan,摇 WANGJiu-he,摇 CHANG Hong (Department of TCM,Affiliated Hospital,InnerMongoliaMedical University,Hohhot010050 China) Abstract:Objective:To study the effects of Mongolian medicine Erweiduzhong Capsule on ovari鄄 ectomied rats Uterine Estrogen Receptor,and search for the mechanism on prevention and treatment postmenopausal osteoporosis of Erweiduzhong Capsule.Methods:We selected 84 female Wistar rats which are 4months old,they were randomly divided into 7 groups:shamed operations group、model group、diethylstilbestrol group、Qianggu capsule group、Erweiduzhong capsule at high、medium、low dose groups,every group had 12 rats. After success of model,we fed rats for 90 days under Normal conditions,the rats in shamed operations group and model group were gavaged separately with Physiological saline.The homologous drugswere gavaged in treatment group.After 90 days,take some blood fr