精氨酸调控仔猪断奶应激的蛋白质双向电泳分析.pdfVIP

精氨酸调控仔猪断奶应激的蛋白质双向电泳分析.pdf

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精氨酸调控仔猪断奶应激的蛋白质双向电泳分析.pdf

AnimalHusbandryand FeedScience.2009。1(4—5):29—33 Responsibleeditor:ZhengDandan Responsibletranslator.ZhangMingming Proteomic-LevelResponsesofEarly--Weaned Piglets to DietaryArginineSupplementation WANGWen-ce。GENGMei·mei,LITie-jun,KONGXiang-feng,CHUWu·ying。GUWan·ting。PANJie,FU De-zhi,FENG Ze-meng,YANG Huan-sheng。HUANG Rui-I_n。YIN Yu·long HunanEngineeringandResearchCenterofAnimalandPoultry ScienceandKeyLaboratoryforAgro·ecologicalProcessesinSubtropicalRegion, InstituteofSubtropiac lAgriculture,ChineseAcademyofSciences,Changsha410125,China Abstract Dietaryargininesupplementation enhancesthe immune statusandprotein synthesisin early·weanedpigs.However.theunderlying mechanismsremainIargelyunknown.Toinvetsigatehow arginineaffectstheexpressionofkeyproteinsthatregulategrowthandnutrienttransport ofjejunum.atotaIof12healthypiglets(21day-old.similarbodyweight,Landraac ×Yorkshire)deliveredbyfoMrSOWSwererandomlydividedinto twogroups.Pigletsinthetestgroupwere rearedwithfeedsupplementedL-arginineataconcentrationof6.0g/kg.whilepigletsinthecontroIgroup werefedwithfeedsupplementedL-alanineataconcentrationof12.3 kg(isonitrogenouscontroI).After7d,iejunummucosaewascollectedand analyzedwiththe2-DPAGEMStechnology.Comparedwiththe∞mroIpigs.argininedecreasedtheIevelsof proteinsthatregulatetheproteinsyn- thesis,intermediarymetabolismandtissuegrowth(similartoanteriorgradient2homolog。cyclophilin_ABHJike,hypothaticalproteinFU39502and tetratricopeptiderepeatdomain16。similartoKIAA0156。mitochondrialATP synthase,hydriontranspo rtingF1complex。betasubunitandalpha-tu- bulinubiquitousisoform 19。prolyI4-hydroxylase.betasubunitprecursor).Inaddition,arginineincreasedtheIevelsofproteinsthatare involvde in proteolysisandjmmuneresponse (PGAM1,TcelJreceptorbetavariable20.membranestereidbindingprotein。sin*Iartomyomesin-1.andchain A,slructureofpigmusclePgkcomplexedwithMgATP).Therefore.arginineinfluencestheimmuneresponseandproteinsynthesismechanismsas wellasimproveseadyweande stre

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